In this work, the concept of lipase cocktail has been proposed in the ultrasound-assisted hydrolysis of coconut oil. Lipase from Thermomyces lanuginosus (TLL), lipase from Rhizomucor miehei (RML), and lipase B from Candida antarctica (CALB) were evaluated as biocatalysts in different combinations. The best conversion (33.66%) was achieved using only RML; however, the best lipase cocktail (75% RML and 25% CALB) proposed by the triangular response surface was used to achieve higher conversions. At the best lipase cocktail, reaction parameters [temperature, biocatalyst content and molar ratio (water/oil)] were optimized by a Central Composite Design, allowing to obtain more than 98% of conversion in the hydrolysis of coconut oil in 3 h of incubation at 37 kHz, 300 W and 45 °C by using 20% of the lipase cocktail (w/w) and a molar ratio of 7.5:1 (water/oil). The lipase cocktail retained about 50% of its initial activity after three consecutive cycles of hydrolysis. To the authors' knowledge, up to date, this communication is the first report in the literature for the ultrasound-assisted hydrolysis of coconut oil catalyzed by a cocktail of lipases. Under ultrasound irradiation, the concept of lipase cocktail was successfully applied, and this strategy could be useful for the other types of reactions using heterogeneous substrates.

中文翻译：

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使用酶混合物制备游离脂肪酸进行声水解。

在这项工作中，已经提出了在超声辅助下水解椰子油的脂肪酶混合物的概念。评价了来自嗜热单胞菌的脂肪酶（TLL），来自米氏根瘤菌的脂肪酶（RML）和来自南极假丝酵母的脂肪酶B（CALB）作为不同组合的生物催化剂。仅使用RML可获得最佳转换率（33.66％）；但是，使用三角响应面建议的最佳脂肪酶混合物（75％RML和25％CALB）可实现更高的转化率。在最佳脂肪酶混合物中，通过中央复合设计优化了反应参数[温度，生物催化剂含量和摩尔比（水/油）]，在孵育3小时后，椰子油水解可实现98％以上的转化率使用20％的脂肪酶混合物（w / w）和7.5的摩尔比在37 kHz，300 W和45°C下 1（水/油）。在连续三个水解周期后，脂肪酶混合物保持其初始活性的约50％。据作者所知，迄今为止，该交流是文献中有关脂肪酶混合物催化的椰子油超声辅助水解的首次报道。在超声辐射下，脂肪酶混合物的概念被成功应用，该策略对于使用异质底物的其他类型的反应可能有用。