ABSTRACT Plant tissues naturally senesce over time. Attempts to improve plant robustness and increase longevity have involved genetic modification, application of synthetic chemicals, and use of beneficial microbes. Recently, culture supernatant from a microalga Chlorella fusca was found to prime innate immunity against Pseudomonas syringae in Arabidopsis thaliana. However, the capacity of Chlorella culture supernatants to prevent or delay aging in higher plants has not been elucidated. In this study, roots of the ornamental flowering plant Erinus alpinus L. were drenched with cell-free supernatants from three Chlorella species. Flower and leaf senescence in E. alpinus was significantly reduced and delayed with all three Chlorella supernatants. Investigations of the mode of action underlying delayed senescence showed that the Chlorella supernatants did not act as a chemical trigger to elicit plant immunity or as a growth-promoting fertilizer in E. alpinus. The mechanisms underlying the anti-aging effects remain undetermined, and several possible hypotheses are discussed. Several Chlorella species are industrially cultivated, and disposal of cell-free supernatant can be economically and environmentally challenging. This study provides a novel method for extending plant lifespan through use of Chlorella supernatant and discusses the potential of using industrial waste supernatants in agriculture and horticulture to reduce reliance on chemical pesticides and genetic modification.

中文翻译：

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植物抗衰老：用无细胞小球藻培养基处理高山鲟的花叶衰老延迟

摘要 植物组织会随着时间自然衰老。提高植物健壮性和延长寿命的尝试涉及基因改造、合成化学品的应用以及有益微生物的使用。最近，发现来自微藻小球藻的培养上清液可以引发针对拟南芥中丁香假单胞菌的先天免疫。然而，小球藻培养上清液在高等植物中预防或延缓衰老的能力尚未阐明。在这项研究中，观赏开花植物 Erinus alpinus L. 的根被三种小球藻的无细胞上清液浸透。所有三种小球藻上清液均显着降低和延迟了高山桉的花和叶衰老。对延迟衰老的作用方式的研究表明，小球藻上清液不能作为引发植物免疫的化学触发剂，也不能作为 E. alpinus 的生长促进肥料。抗衰老作用的潜在机制仍未确定，并讨论了几种可能的假设。几种小球藻属工业化培养，处理无细胞上清液在经济和环境方面都具有挑战性。本研究提供了一种通过使用小球藻上清液延长植物寿命的新方法，并讨论了在农业和园艺中使用工业废物上清液来减少对化学杀虫剂和基因改造的依赖的潜力。抗衰老作用的潜在机制仍未确定，并讨论了几种可能的假设。几种小球藻属工业化培养，处理无细胞上清液在经济和环境方面都具有挑战性。本研究提供了一种通过使用小球藻上清液延长植物寿命的新方法，并讨论了在农业和园艺中使用工业废物上清液来减少对化学杀虫剂和基因改造的依赖的潜力。抗衰老作用的潜在机制仍未确定，并讨论了几种可能的假设。几种小球藻属工业化培养，处理无细胞上清液在经济和环境方面都具有挑战性。本研究提供了一种通过使用小球藻上清液延长植物寿命的新方法，并讨论了在农业和园艺中使用工业废物上清液来减少对化学杀虫剂和基因改造的依赖的潜力。