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Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa
Crystals ( IF 2.7 ) Pub Date : 2020-05-15 , DOI: 10.3390/cryst10050395
Juan Carlos Martínez Espinosa , Raúl Carrera Cerritos , Maria Antonieta Ramírez Morales , Karla Paola Sánchez Guerrero , Rocio Alejandra Silva Contreras , Juan H. Macías

Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics. Silver nanoparticles were synthesized by the green route using the Geranium extract as a reducing agent. The characterization was carried out by the techniques of UV-Vis spectrophotometry, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray emitted photoelectron spectroscopy (XPS) and X-ray diffraction. Nanoparticle diameters between 15 and 50 nm were obtained and the interplanar spaces calculated from the electron diffraction pattern corresponding to a mixture of silver with 4H and FCC structures. To determine the minimum inhibitory concentration of silver nanoparticles (AgNPs) on the Pseudomonas aeruginosa bacteria (ATCC-27853), different concentrations of colloidal solution 0.36, 0.18, 0.09 and 0.05 μg/mL were evaluated as a function of the incubation time, measuring the inhibition halo and colony forming unit (CFU) during 0, 2 and 4 h of incubation. The minimum inhibitory AgNPs concentration (MIC) is 0.36 μg/mL at 0 h while the concentration of 0.18 μg/mL presents a total inhibition of the bacterium after 2 h. For the rest of the dilutions, gradual inhibitions as a function of time were observed. We evaluate the antibacterial effect of silver nanoparticles obtained by a green methodology in Pseudomonas aeruginosa bacteria. Finally, the colloidal nanoparticle solution can be an antibacterial alternative for different biomedical approaches.

中文翻译:

绿色路线获得的银纳米颗粒的表征及其在铜绿假单胞菌细菌中的评价

金属纳米颗粒广泛用于不同领域,例如生物技术和生物医学,例如在药物输送,细菌生长的成像和控制中。银的抗菌作用已被确认为增加细菌对抗生素耐药性的替代方法。使用天竺葵通过绿色路线合成了银纳米颗粒提取物作为还原剂。通过紫外可见分光光度法,透射电子显微镜(TEM),高分辨率透射电子显微镜(HRTEM),X射线发射光电子能谱(XPS)和X射线衍射等技术进行表征。获得了在15和50nm之间的纳米粒径,并且由电子衍射图计算了对应于具有4H和FCC结构的银的混合物的平面间距。确定铜绿假单胞菌对银纳米颗粒(AgNPs)的最小抑制浓度细菌(ATCC-27853),将不同浓度的胶体溶液0.36、0.18、0.09和0.05μg/ mL作为温育时间的函数进行评估,在0、2和4 h内测量抑制晕和菌落形成单位(CFU)孵化。AgNPs的最小抑菌浓度(MIC)在0 h为0.36μg/ mL,而0.18μg/ mL的浓度在2 h后对细菌具有完全抑制作用。对于其余的稀释液,观察到随时间的逐渐抑制作用。我们评估通过绿色方法在铜绿假单胞菌细菌中获得的银纳米颗粒的抗菌作用。最后,胶体纳米粒子溶液可以作为不同生物医学方法的抗菌替代品。
更新日期:2020-05-15
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