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Phytochemical Profile of Capsicum annuum L. cv Senise, Incorporation into Liposomes, and Evaluation of Cellular Antioxidant Activity.
Antioxidants ( IF 7 ) Pub Date : 2020-05-15 , DOI: 10.3390/antiox9050428
Chiara Sinisgalli 1, 2 , Immacolata Faraone 1, 2 , Antonio Vassallo 1 , Carla Caddeo 3 , Faustino Bisaccia 1 , Maria Francesca Armentano 1, 2 , Luigi Milella 1, 2 , Angela Ostuni 1, 2
Affiliation  

Overproduction of oxidants in the human body is responsible for oxidative stress, which is associated with several diseases. High intake of vegetables and fruits can reduce the risk of chronic diseases, as they are sources of bioactive compounds capable of contrasting the free radical effects involved in cancer, obesity, diabetes, and neurodegenerative and cardiovascular diseases. Capsicum annuum L. cv Senise is a sweet pepper that is grown in the Basilicata region (Italy). It is an important source of polyphenols, carotenoids, and capsinoids and can play a key role in human health. In this study, an ethanol extract was obtained from C. annuum dried peppers and the analysis of the phytochemical composition was performed by LC-ESI/LTQ Orbitrap/MS. The extract was incorporated into liposomes, which showed small size (~80 nm), good homogeneity, negative surface charge, and good stability in storage. The biological activity of the extract was evaluated in the human hepatoma (HepG2) cell line, used as model cells. The extract showed no cytotoxic activity and reduced the intracellular reactive oxygen species (ROS) level in stressed cells. The antioxidant activity was further improved when the extract was loaded into liposomes. Moreover, the extract promoted the expression of endogenous antioxidants, such as catalase, superoxide dismutase, and glutathione peroxidase through the Nrf-2 pathway evaluated by RT-PCR.

中文翻译:

辣椒辣椒的植物化学概况,掺入脂质体和评估细胞的抗氧化活性。

人体中氧化剂的过度产生是氧化应激的原因,氧化应激与多种疾病有关。蔬菜和水果的高摄入量可以降低慢性疾病的风险,因为它们是生物活性化合物的来源,能够与癌症,肥胖症,糖尿病以及神经退行性疾病和心血管疾病所涉及的自由基作用进行对比。Capsicum annuum L. cv Senise是一种甜椒,生长在巴西利卡塔地区(意大利)。它是多酚​​,类胡萝卜素和辣椒素的重要来源,并且可以在人类健康中发挥关键作用。在这项研究中,从辣椒干辣椒中提取了乙醇提取物,并通过LC-ESI / LTQ Orbitrap / MS对植物化学成分进行了分析。将提取物掺入脂质体中,脂质体尺寸小(约80 nm),均一性好,负表面电荷,并在储存中具有良好的稳定性。在用作模型细胞的人肝癌(HepG2)细胞系中评估了提取物的生物学活性。提取物显示没有细胞毒活性,并降低了应激细胞的细胞内活性氧(ROS)水平。当将提取物加载到脂质体中时,抗氧化活性进一步提高。此外,提取物通过RT-PCR评估的Nrf-2途径促进内源性抗氧化剂的表达,例如过氧化氢酶,超氧化物歧化酶和谷胱甘肽过氧化物酶。提取物显示没有细胞毒活性,并降低了应激细胞的细胞内活性氧(ROS)水平。当将提取物加载到脂质体中时,抗氧化活性进一步提高。此外,提取物通过RT-PCR评估的Nrf-2途径促进内源性抗氧化剂的表达,例如过氧化氢酶,超氧化物歧化酶和谷胱甘肽过氧化物酶。提取物显示没有细胞毒活性,并降低了应激细胞的细胞内活性氧(ROS)水平。当将提取物加载到脂质体中时,抗氧化活性进一步提高。此外,提取物通过RT-PCR评估的Nrf-2途径促进内源性抗氧化剂的表达,例如过氧化氢酶,超氧化物歧化酶和谷胱甘肽过氧化物酶。
更新日期:2020-05-15
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