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Development of a simple method for sperm cryopreservation of Scombridae fishes in outdoor environments
Aquaculture Research ( IF 2 ) Pub Date : 2020-05-15 , DOI: 10.1111/are.14673
Wataru Kawamura 1 , Ryosuke Yazawa 1 , Reoto Tani 1 , Yutaka Takeuchi 2 , Tetsuro Morita 3 , Hiroyuki Yoshikawa 4 , Goro Yoshizaki 1
Affiliation  

We developed a simple dry shipper method for cryopreserving the sperm of Scombridae fish in outdoor environments. First, we undertook a preliminary study to optimize the sperm cryopreservation conditions using bullet tuna, Auxis rochei (Risso, 1810) sperm. We found that the optimum cryomedium contained 90% foetal bovine serum (FBS) or 300 mM trehalose as an external cryoprotectant and 10% dimethyl sulfoxide (DMSO) as an internal cryoprotectant. Under these optimized conditions, the post‐thaw sperm had a duration of motility of 500 s and a motility rate of >70%. We then performed practical trials of the optimized protocol in various outdoor environments (e.g., fishing boats and ports) using the sperm of five Scombridae species: chub mackerel, Scomber japonicus (Houttuyn, 1782); blue mackerel, S. australasicus (Cuvier, 1832); skipjack tuna, Katsuwonus pelamis (Linnaeus, 1758); longtail tuna Thunnus tonggol, (Bleeker, 1851) and Pacific bluefin tuna, T. orientalis (Temminck & Schlegel, 1844). The post‐thaw sperm of all five of these species had a duration of motility of 650 s and a motility rate of >70%, indicating that this simple method can be used to obtain high‐quality cryopreserved sperm of various Scombridae species in outdoor environments.

中文翻译:

开发一种在室外环境中冷冻保存Scombridae鱼类的精子的简单方法

我们开发了一种简单的干托运人方法,用于在室外环境中冷冻保留Scombridae鱼的精子。首先,我们进行了初步研究,以优化使用子弹金枪鱼Auxis rochei(Risso,1810)精子的精子冷冻保存条件。我们发现最佳的冷冻dium包含90%的胎牛血清(FBS)或300 mM海藻糖作为外部冷冻保护剂,以及10%的二甲基亚砜(DMSO)作为内部冷冻保护剂。在这些优化条件下,解冻后的精子的运动持续时间为500 s,运动率> 70%。然后,我们使用5种com科鱼类的精液在各种室外环境(例如,渔船和港口)中进行了优化方案的实践试验:鲭鱼,S日本ap(豪图因,1782); 蓝鲭鱼,S. australasicus(Cuvier,1832); jack鱼,Katsuwonus pelamis(Linnaeus,1758);长尾金枪鱼Thunnus tonggol(Bleeker,1851)和太平洋蓝鳍金枪鱼T. Orientalis(Temminck&Schlegel,1844)。所有这五个物种的解冻后精子的运动持续时间为650 s,运动率> 70%,这表明该简单方法可用于在室外环境中获得各种Scombridae物种的高质量冷冻保存的精子。 。
更新日期:2020-05-15
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