A Raman‐based, strain‐independent, semi‐automated method is presented that allows the rapid (<3 hours) determination of antibiotic susceptibility of bacterial pathogens isolated from clinical samples. Applying a priori knowledge about the mode of action of the respective antibiotic, we identified characteristic Raman marker bands in the spectrum and calculated batch‐wise weighted sum scores from standardized Raman intensity differences between spectra of antibiotic exposed and nonexposed samples of the same strains. The lead substances for three relevant antibiotic classes (fluoroquinolone ciprofloxacin, third‐generation cephalosporin cefotaxime, ureidopenicillin piperacillin) against multidrug‐resistant Gram‐negative bacteria (MRGN) revealed a high sensitivity and specificity for the susceptibility testing of two Escherichia coli laboratory strains and 12 clinical isolates. The method benefits from the parallel incubation of control and treated samples, which reduces the variance due to alterations in cultivation conditions and the standardization of differences between batches leading to long‐term comparability of Raman measurements.

中文翻译：

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使用特定的拉曼标记条带自动快速鉴定针对酰基脲体青霉素，头孢菌素和氟喹诺酮类药物的多重耐药性大肠杆菌。

提出了一种基于拉曼，应变独立，半自动化的方法，该方法可以快速（<3小时）测定从临床样品中分离出的细菌病原体的抗生素敏感性。应用先验在了解了各自抗生素的作用方式后，我们确定了光谱中的特征拉曼标记带，并根据暴露于同一菌株的抗生素样品与未暴露样品之间的光谱之间的标准化拉曼强度差，计算了分批加权总和得分。三种针对多药耐药革兰氏阴性菌（MRGN）的相关抗生素类别的主要物质（氟喹诺酮环丙沙星，第三代头孢菌素头孢噻肟，尿道青霉素哌拉西林）显示出对两种大肠杆菌的敏感性测试的高敏感性和特异性实验室菌株和12种临床分离株。该方法得益于对照样品和处理样品的平行孵育，可减少由于培养条件的变化和批次之间差异的标准化导致的差异，从而可实现拉曼测量的长期可比性。