The electrochemical sensing of clonazepam in samples of unprocessed saliva is reported, using commercially available reduced graphene oxide (rGO) screen printed electrodes (SPEs). The rGO electrodes were modified with self‐assembled lubricin (LUB) antiadhesive coatings that function as a size‐selective molecular sieve that partitions small molecule analytes from large fouling molecules at the electrode‐fluid interface. By mitigating the adverse effects of protein fouling, these LUB coatings enable direct, reliable, and quantitative electrochemical measurements of clonazepam concentrations in untreated saliva, which was not possible with uncoated rGO electrodes in identical tests. The proposed assay design, which is suitable for disposable point‐of‐care diagnostics requires no sample pre‐treatment and shows a linear detection response over a concentration range of 25–250 nM with a correlation coefficient of R²=0.987 and a limit of detection (LoD) of 16.67 nM. The swab‐based sample acquisition method paired with the antifouling properties of the LUB coatings provide a cost effective and practical biosensing platform for assessing electroactive drug concentrations in saliva in a point‐of‐care setting.

中文翻译：

---

一种简单的电化学拭子测定法，用于快速定量卢比霉素防污涂料中未加工唾液中的氯硝西am

据报道，使用可商购的还原氧化石墨烯（rGO）丝网印刷电极（SPEs）对未经处理的唾液样品中的氯硝西am进行电化学检测。rGO电极经过了自组装的lubricin（LUB）抗粘涂层的修饰，该涂层起着大小选择分子筛的作用，该筛子将小分子分析物与大污染分子在电极-流体界面处隔开。通过减轻蛋白质结垢的不利影响，这些LUB涂层可以对未经处理的唾液中的氯硝西concentrations浓度进行直接，可靠和定量的电化学测量，而在相同的测试中使用未涂覆的rGO电极是不可能的。拟议的分析设计，² = 0.987，检测限（LoD）为16.67 nM。基于拭子的样品采集方法与LUB涂层的防污性能相结合，为现场护理环境中唾液中电活性药物浓度的评估提供了一种经济有效的实用生物传感平台。