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Doxorubicin-Conjugated Iron Oxide Nanoparticles: Surface Engineering and Biomedical Investigation.
ChemPlusChem ( IF 3.4 ) Pub Date : 2020-05-15 , DOI: 10.1002/cplu.202000360
Viktoriia Oleksa 1 , Hana Macková 1 , Vitalii Patsula 1 , Aneta Dydowiczová 2 , Olga Janoušková 2 , Daniel Horák 1
Affiliation  

Development of therapeutic systems to treat glioblastoma, the most common and aggressive brain tumor, belongs to priority tasks in cancer research. We have synthesized colloidally stable magnetic nanoparticles (D h=336 nm) coated with doxorubicin (Dox) conjugated copolymers of N,N ‐dimethylacrylamide and either N ‐acryloylglycine methyl ester or N ‐acryloylmethyl 6‐aminohexanoate. The terminal carboxyl groups of the copolymers were reacted with alendronate by carbodiimide formation. Methyl ester groups were then transferred to hydrazides for binding Dox by a hydrolytically labile hydrazone bond. The polymers were subsequently bound on the magnetic nanoparticles through bisphosphonate terminal groups. Finally, the anticancer effect of the Dox‐conjugated particles was investigated using the U‐87 glioblastoma cell line in terms of particle internalization and cell viability, which decreased to almost zero at a concentration of 100 μg of particles per ml. These results confirmed that poly(N,N ‐dimethylacrylamide)‐coated magnetic nanoparticles can serve as a solid support for Dox delivery to glioblastoma cells.

中文翻译:

阿霉素结合的氧化铁纳米粒子:表面工程和生物医学研究。

治疗胶质母细胞瘤(最常见和侵略性脑瘤)的治疗系统的开发属于癌症研究的优先任务。我们合成了涂有N,N-二甲基丙烯酰胺和N-丙烯酰基甘氨酸甲酯或N的阿霉素(Dox)共轭共聚物的胶体稳定磁性纳米颗粒(D h = 336 nm)丙烯酰基甲基6-氨基己酸酯 通过碳二亚胺的形成,使共聚物的末端羧基与阿仑膦酸酯反应。然后将甲酯基团通过水解不稳定的键转移到酰肼上,以结合Dox。随后,聚合物通过双膦酸酯末端基团结合在磁性纳米颗粒上。最后,使用U-87胶质母细胞瘤细胞系研究了Dox缀合颗粒的抗癌作用,涉及颗粒内在化和细胞活力,当每毫升颗粒浓度为100μg时,其几乎降至零。这些结果证实了聚(N,N-二甲基丙烯酰胺)涂层的磁性纳米颗粒可以作为Dox递送至胶质母细胞瘤细胞的固体支持物。
更新日期:2020-05-15
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