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Bacterial Cell Display as a Robust and Versatile Platform for Engineering Low-Affinity Ligands and Enzymes.
ChemBioChem ( IF 3.2 ) Pub Date : 2020-05-15 , DOI: 10.1002/cbic.202000203
Eszter Csibra 1, 2 , Marleen Renders 3, 4 , Vitor B Pinheiro 1, 3, 5
Affiliation  

Displaying enzymes and low‐affinity binding proteins on the surface of the bacterial cell: A SNAP reporter together with the function‐dependent labelling of the cell with cholesterol‐linked DNA, allows systematic optimisation of cell display, making it a robust and flexible platform for the systematic evolution of XNA molecular biology.
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中文翻译:

细菌细胞展示作为工程低亲和力配体和酶的稳健且多功能的平台。

在细菌细胞表面展示酶和低亲和力结合蛋白:SNAP 报告基因与胆固醇连接 DNA 的细胞功能依赖性标记一起,可以系统地优化细胞展示,使其成为一个强大而灵活的平台XNA分子生物学的系统进化。
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更新日期:2020-05-15
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