An 11G nucleotide repeat in the 3′ UTR of FAM174A was recently postulated as a risk allele with a dominant mode of inheritance for equine metabolic syndrome (EMS) and laminitis status in Arabian horses. The objective of this project was to evaluate this hypothesis in a large and diverse across‐breed population. A total of 301 ponies, 292 Morgans, 64 Arabians, 49 Tennessee Walking Horses and 59 Quarter Horses were genotyped for six observed G repeat alleles in the FAM174A 3′ UTR. Phenotype data included laminitis status, baseline insulin, glucose, non‐esterified fatty acids, triglycerides, adiponectin, leptin, ACTH, insulin and glucose post oral sugar test, and two proxies for insulin resistance. The 11G allele frequencies were 18.8, 6.9, 1.8, 0.2 and 0.0% in the Arabians, Tennessee Walkers, ponies, Morgans and Quarter Horses respectively. Association analyses between FAM174A genotype and EMS phenotypes, and between allele count and EMS phenotypes, identified no statistically significant associations. When a dominant effect for the 11G allele was evaluated, a statistically significant association with adiponectin levels was identified in the ponies, and pairwise comparisons revealed that the estimated marginal means were higher in ponies with the 11G allele vs. alternative alleles (i.e. the allele had a protective effect). In conclusion, our data do not support the FAM174A 11G allele as a risk allele for EMS in our studied breeds.

中文翻译：

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FAM174A 11G等位基因评估为马代谢综合征的风险等位基因。

FAM174A 3'UTR中的一个11G核苷酸重复最近被认为是风险等位基因，具有阿拉伯马马代谢综合症（EMS）和椎板炎状态的显性遗传方式。该项目的目的是在众多多样的跨种群中评估这一假设。对FAM174A中6个观察到的G重复等位基因进行了基因分型，共301个小马，292个Morgans，64个阿拉伯人，49个田纳西州步行马和59个四分之一马。3'UTR。表型数据包括椎间盘炎状态，基线胰岛素，葡萄糖，非酯化脂肪酸，甘油三酸酯，脂联素，瘦素，ACTH，口服糖测试后的胰岛素和葡萄糖，以及两个胰岛素抵抗指标。阿拉伯人，田纳西州沃克，小马，摩根和四分之一马的11G等位基因频率分别为18.8％，6.9％，1.8％，0.2％和0.0％。FAM174A之间的关联分析基因型和EMS表型，以及等位基因计数和EMS表型之间，没有发现统计学上的显着关联。当评估11G等位基因的显性作用时，在小马中与脂联素水平存在统计学上的显着关联，并且成对比较显示，与11G等位基因相比，小马中估计的边际均值较高（即等位基因具有保护作用）。总之，我们的研究数据不支持FAM174A 11G等位基因作为EMS的风险等位基因。