Cannabis sativa plants growing nearby sugarcane fields affected with grassy shoot disease at Muzaffarnagar, Uttar Pradesh, India, showed severe witches’ broom symptoms. The DNA from symptomatic Cannabis and sugarcane samples was isolated and subjected to nested PCR assays with universal phytoplasma-specific primer pairs (P1/P7 and R16F2n/R16R2). A DNA fragment of ~ 1.2 kbp was amplified in all the symptomatic samples of sugarcane and Cannabis with R16F2n/R16R2 primer pair but was absent in non-symptomatic samples. Pairwise sequence comparison, phylogeny and in silico RFLP analysis of 16S rDNA sequences confirmed the identity of a rice yellow dwarf phytoplasma group-related strain (16SrXI-B) associated with Cannabis which suggested its role as potential natural reservoir of SCGS phytoplasma in nature.

中文翻译：

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大麻：印度潜在的甘蔗草芽植物枯萎病水库

印度北方邦穆扎法纳那（Muzaffarnagar）上生长着草grass病的甘蔗田附近种植的大麻植物显示出严重的女巫扫帚症状。分离有症状大麻和甘蔗样品中的DNA，并使用通用的植物质体特异性引物对（P1 / P7和R16F2n / R16R2）进行嵌套式PCR分析。使用R16F2n / R16R2引物对，在所有有症状甘蔗和大麻样品中扩增出约1.2 kbp的DNA片段，但在无症状样品中却不存在。对16S rDNA序列的成对序列比较，系统发育和计算机RFLP分析证实了与大麻有关的水稻黄矮化质体群相关菌株（16SrXI-B）的身份 这表明它是自然界中SCGS植物质体的潜在天然贮藏库。