Visacane, CIRAD’s sugarcane quarantine program (http://visacane.cirad.fr/en/), covers three main quarantine procedures: detection of pests and pathogens, elimination of pests and pathogens, and transfer of pest- and pathogen-free planting material. While we aim at improving sugarcane adaptability, stability, and sustainability through Visacane, sugarcane clones and related wild species with high levels of genetic diversity and originating from multiple sources are quarantined at Montpellier (France) to be sent to Visacane’s partners. From 2013 to 2017, 2,830 batches of disease-free cuttings from 435 sugarcane clones were transferred to 42 partners of Visacane. Our main goal was to foster worldwide distribution of pest- and pathogen-free planting material and to guarantee breeder’s rights. The virome of sugarcane was recently investigated using viral metagenomics, and the genetic diversity of three uncharacterized sugarcane viruses (sugarcane white streak virus, sugarcane striate virus, and sugarcane mild mosaic virus) was assessed for improved diagnosis. Hence, new protocols to detect these three viruses were developed and validated. An additional detection protocol targeting sugarcane bacilliform virus, a virus that was up to now not considered as a quarantine organism by many countries and undiagnosed in quarantine, was also developed. These issues are essential to guarantee the health status of the planting material and to increase the reliability of Visacane’s sugarcane germplasm.

中文翻译：

---

结合使用病毒超基因组学和基于PCR的方法，改进CIRAD甘蔗检疫中心的病毒检测

CIRAD的甘蔗检疫计划Visacane（http://visacane.cirad.fr/en/）涵盖了三个主要检疫程序：有害生物和病原体的检测，有害生物和病原体的消除以及无有害生物和无病原体的种植材料的转移。尽管我们旨在通过Visacane改善甘蔗的适应性，稳定性和可持续性，但蒙彼利埃（法国）隔离了具有高遗传多样性和多种来源的甘蔗克隆和相关野生物种，并将其发送给Visacane的合作伙伴。从2013年到2017年，来自435个甘蔗克隆的2830批无病插条被转移到Visacane的42个合作伙伴。我们的主要目标是促进无害和无病原体种植材料的全球分布，并确保育种者的权利。最近使用病毒宏基因组学研究了甘蔗的病毒，并评估了三种未表征的甘蔗病毒（甘蔗白条病毒，甘蔗纹状体病毒和甘蔗轻度花叶病毒）的遗传多样性，以改善诊断。因此，开发并验证了检测这三种病毒的新协议。还开发了针对甘蔗杆菌状病毒的另一种检测方案，该病毒迄今尚未被许多国家视为检疫生物，但在检疫中未得到诊断。这些问题对于保证种植材料的健康状况以及提高Visacane甘蔗种质的可靠性至关重要。并评估了甘蔗轻度花叶病毒）以改善诊断。因此，开发并验证了检测这三种病毒的新协议。还开发了针对甘蔗杆菌型病毒的另一种检测方案，该病毒迄今尚未被许多国家视为检疫生物，但在检疫中未得到诊断。这些问题对于保证种植材料的健康状况以及提高Visacane甘蔗种质的可靠性至关重要。并评估了甘蔗轻度花叶病毒）以改善诊断。因此，开发并验证了检测这三种病毒的新协议。还开发了针对甘蔗杆菌状病毒的另一种检测方案，该病毒迄今尚未被许多国家视为检疫生物，但在检疫中未得到诊断。这些问题对于保证种植材料的健康状况以及提高Visacane甘蔗种质的可靠性至关重要。