Abstract

The tea geometrid Ectropis grisescens is an important pest of tea plant (Camellia sinensis (L.) O. Kuntze). It feeds on the new leaves and tender stems of tea plants, causing serious damage. The green leaf volatile (Z)-3-hexenol (z3HOL) can effectively activate the defense of tea against E. grisescens in the laboratory. In this study, z3HOL was confirmed to activate the tea mitogen-activated protein kinase and jasmonic acid (JA) burst in the laboratory. We investigated effects of z3HOL on the defense responses of tea plants under field conditions. Our results showed that tea plants exposed to z3HOL showed higher levels of JA and polyphenol oxidase (PPO) and emitted increased quantities of volatile organic compounds (VOCs) compared to non-exposed plants. Less E. grisescens larvae were observed in z3HOL-treated plots and less damage was found in z3HOL-treated plants than control. The number of Apanteles sp. and the parasitism of E. grisescens were also enhanced by z3HOL. These findings indicate that synthetic z3HOL can triggers tea defense responses against E. grisescens in the field, and that it could be used as a chemical elicitor to enhance biological control of tea geometrid in the future.

中文翻译：

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（Z）-3-己烯醇在田间诱导茶抗灰c

摘要

茶几何形状的Ectropis grisescens是茶树的重要害虫（山茶（L.）O。Kuntze）。它以茶树的新叶和嫩茎为食，造成严重破坏。绿叶挥发物（Z）-3-己烯醇（z3HOL）可以有效地激活茶对E. grisescens的防御在实验室里。在这项研究中，在实验室中证实了z3HOL可激活茶中的丝裂原活化蛋白激酶和茉莉酸（JA）爆发。我们调查了田间条件下z3HOL对茶树防御反应的影响。我们的结果表明，与未暴露的植物相比，暴露于z3HOL的茶树表现出较高的JA和多酚氧化酶（PPO）水平，并散发了更多的挥发性有机化合物（VOC）。少E. grisescens在z3HOL处理的地块，观察幼虫和在比对照z3HOL处理的植物中发现较少的损害。Apanteles sp。的数目。和E. grisescens的寄生也通过z3HOL进行了增强。这些发现表明，合成的z3HOL可以触发田间对格氏大肠杆菌的茶防御反应，并且它可以用作化学激发剂，以增强将来对几何茶的生物学控制。