Abstract

In this study, the response of the chloroplastic antioxidant system of the halophyte Cakile maritima Scop. and its tolerance to NaCl stress have been studied using purified chloroplasts. Seedlings were grown in different salt concentrations (0, 100, 200 and 400 mmol/L NaCl) and plants were harvested after 40 days. Isolated chloroplasts were purified by centrifugation in density-gradients of Percoll. The evaluation of the oxidative stress was analysed measuring lipid peroxidation, carbonyl protein, \({\text{O}}_{2}^{ - }\) and H₂O₂ contents and the antioxidant status by measurement of the activities of superoxide dismutase, catalase, peroxidase and enzymes of the ascorbate–glutathione cycle as well as the antioxidants ascorbate and glutathione, in the purified chloroplasts. Results revealed that the best growth response of C. maritima under moderate salt stress was associated with a low oxidative stress, the highest activities of SOD, POD and APX and the highest glutathione content. The interaction between the water status of the plant and mineral nutrition seems to be strongly involved in the plant performance under salinity.

中文翻译：

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叶绿体对耐盐生盐藻的耐盐性的影响

摘要

在这项研究中，盐生植物Cakile maritima Scop的叶绿体抗氧化剂系统的响应。已经使用纯化的叶绿体研究了其对NaCl胁迫的耐受性。在不同的盐浓度（0、100、200和400 mmol / L NaCl）中培养幼苗，并在40天后收获植物。通过在Percoll的密度梯度中离心来纯化分离的叶绿体。通过测量脂质过氧化，羰基蛋白，\（{\ text {O}} _ {2} ^ {-} \）和H ₂ O _2来分析氧化应激的评估通过测量纯化叶绿体中超氧化物歧化酶，过氧化氢酶，过氧化物酶和抗坏血酸-谷胱甘肽循环酶的活性以及抗氧化剂抗坏血酸和谷胱甘肽的含量和抗氧化剂状态。结果表明，中度盐胁迫下海藻的最佳生长响应与低氧化应激，SOD，POD和APX活性最高以及谷胱甘肽含量最高有关。在盐度下，植物的水分状态与矿质营养之间的相互作用似乎与植物的性能密切相关。