Abstract

Plant root architecture modulates during developmental stages and adjusts with the environmental condition. The cytosolic calcium which is a ubiquitous secondary messenger in all eukaryotes strongly affects the root system in Arabidopsis thaliana (L.) Heynh. We proposed that calcium chloride gradients affect PIN2 expression, which in term modulates root architecture and lateral root emergence. In the present study, the root development of PIN2 overexpressing lines of A. thaliana were investigated on different CaCl₂ concentrations. This study found that the abundance of PIN2 protein has a direct effect on the root curvature with respect to CaCl₂ gradient. In the presence of low concentration of CaCl₂, PIN2 protein is stabilized and its subsequent accumulation lead to straight root architecture. However, as the CaCl₂ concentration were increased, PIN2 protein destabilized and subsequently degraded which in turn showed a wavy root phenotype. On the other hand, different concentrations of CaCl₂ did not show any effect on PIN2 gene at transcript level.

中文翻译：

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拟南芥初级根构型和侧根萌发中Ca 2+ 2盐的信号传递

摘要

植物的根系结构在发育阶段进行调节，并根据环境条件进行调节。胞质钙是所有真核生物中普遍存在的次级信使，强烈影响拟南芥（L.）Heynh的根系。我们提出氯化钙梯度会影响PIN2的表达，而PIN2的表达会调节根的结构和侧根的出苗。在本研究中，在不同的CaCl ₂浓度下研究了拟南芥PIN2过表达品系的根发育。这项研究发现，相对于CaCl ₂，PIN2蛋白的丰度直接影响根曲率梯度。在低浓度的CaCl ₂存在下，PIN2蛋白稳定，其随后的积累导致直根结构。然而，随着CaCl ₂浓度的增加，PIN2蛋白不稳定并随后降解，进而显示出波浪状的根表型。另一方面，不同浓度的CaCl ₂在转录水平上对PIN2基因没有任何影响。