Role of rhizobacteria and zinc (Zn) was investigated in the management of charcoal rot disease in mungbean [Vigna radiata (L.) Wilczek] caused by Macrophomina phaseolina (Tassi) Goid. In vitro, screening tests with eight rhizobacteria [Bacillus subtilis (FCBP-0324), B. subtilis (FCBP-0189), Rhizobacter daucus (FCBP-0450), Azospirillum brasilense (FCBP-0025), Azospirillum lipoferum (FCBP-0022), Pseudomonas malophilia (FCBP-0099), Pseudomonas florescense (FCBP-0083) and Ochrobactrum ciceri (FCBP-0727)] were conducted against M. phaseolina and FCBP-0727 were found as the most effective biocontrol agent. Molecular analyses of 16S rDNA combined with cultural and biochemical analyses confirmed FCBP-0727 identification (GeneBank Accession No. LC415039). Cell-free culture filtrate (CFCF) and cell culture of O. ciceri were separated and antifungal trials of both substrates indicated inhibition in mycelial growth and suppression in sclerotia formation, although the CFCF appeared to be more destructive against the pathogen. Ethyl-acetate and chloroform extracts of bacterial secondary metabolites completely halted the growth of M. phaseolina. The GC–MS analysis of CFCF of chloroform extract proved to be rich sources of bioactive fungicide like phthalates, adipic acid, propanoic acid, and linoleic acid. Likewise, CFCF of ethyl acetate also exhibited important organic compounds like phthalates, diisopropylglycol and octasiloxan. Pot experiment revealed that soil inoculation with O. ciceri in combination with Zn (2.5 mg/kg) protected mungbean plants against M. phaseolina through improving photosynthetic pigment, total protein content and activities of antioxidant enzymes (catalase, peroxidase and polyphenol oxidase). The present study will open new vistas for biological management of charcoal rot disease of mungbean using a combination of rhizobacteria and Zn.

中文翻译：

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通过使用生防控O菜和锌防治绿豆的腐烂病。

研究了根际细菌和锌（Zn）在处理由菜豆（Macphophomina phaseolina（Tassi）Goid）引起的绿豆[ Vigna radiata（L.）Wilczek]木炭腐烂病中的作用。在体外，对八种根瘤菌进行了筛查测试[枯草芽孢杆菌（FCBP-0324），枯草芽孢杆菌（FCBP-0189），道康根菌（FCBP-0450），巴西固氮螺菌（FCBP-0025），脂氮固氮螺菌（FCBP-0022），针对菜豆分枝杆菌进行了嗜假单胞菌嗜血假单胞菌（FCBP-0099），弗氏假单胞菌（FCBP-0083）和and形棒菌（FCBP-0727）] 。发现FCBP-0727和FCBP-0727是最有效的生物防治剂。结合文化和生化分析对16S rDNA进行分子分析，证实了FCBP-0727的鉴定（GeneBank登录号LC415039）。分离了无细胞培养物滤液（CFCF）和蝉菌的细胞培养物，两种底物的抗真菌试验均表明菌丝体生长受到抑制，菌核形成受到抑制，尽管CFCF似乎对病原体更具破坏性。细菌次生代谢产物的乙酸乙酯和氯仿提取物完全阻止了菜豆分枝杆菌的生长。氯仿提取物的​​CFCF的GC-MS分析证明是生物活性杀菌剂的丰富来源，例如邻苯二甲酸盐，己二酸，丙酸和亚油酸。同样，乙酸乙酯的CFCF也显示出重要的有机化合物，例如邻苯二甲酸酯，二异丙基二醇和八硅氧烷。盆栽试验表明，土壤玉米蜡菌与锌（2.5毫克/千克）的组合接种可通过改善光合色素，总蛋白含量和抗氧化酶（过氧化氢酶，过氧化物酶和多酚氧化酶）的活性来保护绿豆免受菜豆分枝杆菌的侵害。本研究将利用根瘤菌和锌的组合，为生物防治绿豆腐烂病开辟新的前景。