This work aimed to synthesize silver nanoparticles (AgNPs) using cashewnut shell extract (CNSE) and evaluate its antibacterial and anti-biofilm activities against Staphylococcus aureus. CNSE showed the presence of isorhamnetin as the major phytochemical compound. The size, shape, morphology and stability of synthesized CNSE-AgNPs were investigated using ultraviolet–visible spectroscopy (peak at 433 nm), zeta potential (− 24.2 mV), field emission scanning electron microscopy (89.1–100.8 nm) and transmission electron microscopy (Spherical shape with diameter of 3–25 nm). X-ray diffraction data indicated the presence of silver in crystalline form (2-theta values of 38, 54 and 77). CNSE-AgNPs showed strong antibacterial activity with zone of inhibition (15 ± 0.06 to 20 ± 0.17 mm) and minimum inhibitory concentration (MIC) (7.81–31.25 μg/ml) against Staphylococcus aureus strains isolated from fresh cow milk samples, methicillin sensitive Staphylococcus aureus (MSSA) (ATCC-25923) and methicillin resistant Staphylococcus aureus (MRSA) (ATCC-700699). CNSE-AgNPs exhibited higher level of nucleotide leakage through membrane disruption (8.97–10.76 folds), reactive oxygen species generation (1.92–3.3 folds), malondialdehyde production (16.85–30.39%) and biofilm inhibition (54.82–78.25%) when compared to untreated Staphylococcus aureus. CNSE-AgNPs was found to effectively eradicate the artificially inoculated Staphylococcus aureus in milk model within 4th h.

这项工作旨在使用腰果壳提取物（CNSE）合成银纳米颗粒（AgNP），并评估其对金黄色葡萄球菌的抗菌和抗生物膜活性。。CNSE显示异鼠李素是主要的植物化学化合物。合成的CNSE-AgNPs的大小，形状，形态和稳定性使用紫外可见光谱法（峰值在433 nm），ζ电位（− 24.2 mV），场发射扫描电子显微镜（89.1–100.8 nm）和透射电子显微镜进行了研究。 （直径为3–25 nm的球形）。X射线衍射数据表明存在结晶形式的银（2-θ值为38、54和77）。CNSE-AgNPs对分离自新鲜牛奶样品，对甲氧西林敏感的葡萄球菌的金黄色葡萄球菌菌株具有强大的抗菌活性，并具有抑制区（15±0.06至20±0.17 mm）和最小抑制浓度（MIC）（7.81–31.25μg/ ml）。金黄色（MSSA）（ATCC-25923）和耐甲氧西林的金黄色葡萄球菌（MRSA）（ATCC-700699）。与之相比，CNSE-AgNPs通过膜破坏（8.97–10.76倍），活性氧生成（1.92–3.3倍），丙二醛生成（16.85–30.39％）和生物膜抑制（54.82–78.25％）表现出更高水平的核苷酸泄漏。未经处理的金黄色葡萄球菌。研究发现，CNSE-AgNPs可在4 h内有效根除牛奶模型中人工接种的金黄色葡萄球菌。