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Production, purification, and characterization of cellulase from Acinetobacter junii GAC 16.2, a novel cellulolytic gut isolate of Gryllotalpa africana, and its effects on cotton fiber and sawdust
Annals of Microbiology ( IF 3 ) Pub Date : 2020-05-14 , DOI: 10.1186/s13213-020-01569-6
Sandipan Banerjee , Tushar Kanti Maiti , Raj Narayan Roy

The study aims to search for potent cellulase producer from the gut of Gryllotalpa africana as well as to characterize and determine the effect of the purified enzyme on the cellulosic waste materials. The potent cellulolytic strain was identified through morphological, biochemical, physiological, and molecular characterization like 16S rRNA and fatty acid methyl ester profile. After the optimization of cellulase production, the enzyme was purified through DEAE-Sepharose column chromatographic separation. The molecular weight of the purified enzyme was determined by SDS-PAGE analysis. The purified enzyme was characterized in terms of its activity and its effect on cotton fiber, and sawdust was also studied. The selected potent strain GAC 16.2 was identified as Acinetobacter junii that was capable to produce enhanced cellulase (112.38 ± 0.87 U/ml) at standardized optimum fermentation conditions. The molecular weight of the purified enzyme was determined as 55 kDa. The utmost activity of the purified enzyme was detected pH 7.0, temperature 50 °C, and in the presence of metal ions like Mg2+ and Mn2+. The substantive degradation of cotton fiber and sawdust has been observed in a reasonably short period. Purified cellulase from the selected isolate A. junii GAC16.2, a gut isolate of G. africana, has the potentiality to degrade cellulosic substances. This property can make the isolate a potent candidature for industrial application, as well as an effective biotechnological tool for environmental monitoring through cellulosic waste management.

中文翻译:

新型非洲灰霉菌纤维素分解肠分离物Jun Acinetobacter junii GAC 16.2的纤维素酶的生产,纯化和特​​性及其对棉纤维和木屑的影响

该研究旨在从非洲灰线耳的肠道中寻找有效的纤维素酶生产者,并表征和确定纯化的酶对纤维素废料的影响。通过形态,生化,生理和分子表征(如16S rRNA和脂肪酸甲酯分布图)鉴定了有效的纤维素分解菌株。优化纤维素酶生产后,通过DEAE-Sepharose柱色谱分离法纯化酶。通过SDS-PAGE分析确定纯化的酶的分子量。根据其活性及其对棉纤维的作用来表征纯化的酶,并对木屑进行了研究。选定的强效GAC 16.2菌株被鉴定为能够产生增强的纤维素酶(112.38±0。87 U / ml)在标准的最佳发酵条件下。纯化的酶的分子量确定为55kDa。在pH值为7.0,温度为50°C且存在金属离子(例如Mg2 +和Mn2 +)的情况下,检测到了纯化酶的最大活性。在相当短的时间内就观察到了棉纤维和锯末的大量降解。从选定的分离物A. junii GAC16.2(一种非洲假单胞菌的肠道分离物)中纯化的纤维素酶具有降解纤维素物质的潜力。此特性可以使分离物成为工业应用的有效候选者,并成为通过纤维素废物管理进行环境监测的有效生物技术工具。在pH值为7.0,温度为50°C且存在金属离子(例如Mg2 +和Mn2 +)的情况下,检测到了纯化酶的最大活性。在相当短的时间内就观察到了棉纤维和锯末的大量降解。从选定的分离物A. junii GAC16.2(一种非洲假单胞菌的肠道分离物)中纯化的纤维素酶具有降解纤维素物质的潜力。此特性可以使分离物成为工业应用的有效候选者,并成为通过纤维素废物管理进行环境监测的有效生物技术工具。在pH值为7.0,温度为50°C且存在金属离子(例如Mg2 +和Mn2 +)的情况下,检测到了纯化酶的最大活性。在相当短的时间内就观察到了棉纤维和锯末的大量降解。从选定的分离物A. junii GAC16.2(一种非洲假单胞菌的肠道分离物)中纯化的纤维素酶具有降解纤维素物质的潜力。此特性可以使分离物成为工业应用的有效候选者,并成为通过纤维素废物管理进行环境监测的有效生物技术工具。非洲棉的肠道分离物具有降解纤维素物质的潜力。此特性可以使分离物成为工业应用的有效候选者,并成为通过纤维素废物管理进行环境监测的有效生物技术工具。非洲棉的肠道分离物具有降解纤维素物质的潜力。此特性可以使分离物成为工业应用的有效候选者,并成为通过纤维素废物管理进行环境监测的有效生物技术工具。
更新日期:2020-05-14
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