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Psittacid herpesvirus 3 infection in rose-ringed parakeets in southern Brazil.
The Journal of Veterinary Diagnostic Investigation ( IF 1.5 ) Pub Date : 2020-05-01 , DOI: 10.1177/1040638720913075
Laurete Murer 1, 2 , Marília B Ribeiro 1, 2 , Glaucia D Kommers 1, 2 , Mauro P Soares 1, 2 , Juliana F Cargnelutti 1, 2 , Eduardo F Flores 1, 2 , Maristela Lovato 1, 2
Affiliation  

We diagnosed disease caused by psittacid herpesvirus 3 (PsHV-3), a novel psittacid pathogen, in rose-ringed parakeets (Psittacula krameri) housed in an exotic psittacine breeding colony in southern Brazil. The disease affected several adult birds. Clinical signs included apathy, tachypnea, and wheezing. Four birds were autopsied, and sections of lungs and liver were examined histologically and by electron microscopy (EM), revealing pulmonary congestion, bronchopneumonia, or multifocal necrosis of tertiary bronchi, with syncytial cells and eosinophilic intranuclear inclusion bodies. Viral particles morphologically compatible with herpesviruses were observed by EM in lung sections. PCR with pan-herpesvirus primers performed on total DNA extracted from paraffinized tissue resulted in a 278-bp product. Sequencing of the amplicon revealed 93% nucleotide identity with a PsHV-3 sequence available in GenBank. Phylogenetic analysis grouped the obtained sequence with the only PsHV-3 DNA polymerase gene sequence available (GenBank accession JX028240) and separated the sequence from psittacid herpesviruses 1 and 2. The clinical, pathologic, and molecular findings support the association of PsHV-3 with pneumonia found in these rose-ringed parakeets in southern Brazil.

中文翻译:

巴西南部玫瑰环鹦鹉中的Psittacid疱疹病毒3感染。

我们在巴西南部一个异域的鹦鹉螺繁殖地中饲养的玫瑰环鹦鹉(Psittacula krameri)中,诊断出了由一种新型的鹦鹉螺病原菌致癌性疱疹病毒3(PsHV-3)引起的疾病。该病影响了几只成年鸟类。临床体征包括冷漠,呼吸急促和喘息。对四只鸟进行了尸检,并通过组织学和电子显微镜(EM)检查了肺和肝脏的部分,发现肺充血,支气管肺炎或三级支气管多灶性坏死,并伴有合胞细胞和嗜酸性核内包涵体。EM在肺切片中观察到与疱疹病毒形态相容的病毒颗粒。用全疱疹病毒引物对从石蜡化组织中提取的总DNA进行PCR,可得到278 bp的产物。扩增子的测序显示与GenBank中可用的PsHV-3序列具有93%的核苷酸同一性。系统发育分析将获得的序列与唯一可用的PsHV-3 DNA聚合酶基因序列(GenBank登录号JX028240)进行了分组,并将该序列与灵芝疱疹病毒1和2分离。临床,病理学和分子生物学发现均支持PsHV-3与肺炎的关联在巴西南部的这些玫瑰花环长尾小鹦鹉中发现。
更新日期:2020-05-13
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