High-throughput enzyme screening for desired functionality is highly demanded. This paper utilizes a newly developed microfluidic pneumatic printing platform for high-throughput enzyme screening applications. The novel printing platform can achieve distinct features including a disposable cartridge, which avoids crosstalk; a flexible cartridge design, allowing for integration of multiple channels; and fast printing speed with submicroliter spot size. Moreover, a polydimethylsiloxane (PDMS)-based sandwich structure has been proposed and used during the printing and imaging, which can lead to better results, including reduced evaporation as well as a uniform light path during imaging. Using this microfluidic pneumatic printed PDMS sandwiched microdroplet array platform, we have demonstrated the capability of high-throughput generation of a combinatorial droplet array with concentration and volume gradients. Furthermore, the potential for enzymatic study has been validated by quantified cellulose reaction implemented with the printing platform.

中文翻译：

---

用于高通量酶促反应和筛选的微流体气动印刷夹心微滴阵列。

非常需要高通量酶筛选所需的功能。本文利用新开发的微流体气动打印平台进行高通量酶筛选应用。新颖的打印平台可以实现独特的功能，包括一次性墨盒，避免串扰；灵活的墨盒设计，允许集成多个通道；和亚微升光斑尺寸的快速打印速度。此外，基于聚二甲基硅氧烷 (PDMS) 的夹心结构已被提出并在打印和成像过程中使用，这可以带来更好的结果，包括减少蒸发以及成像过程中的均匀光路。使用这种微流体气动印刷 PDMS 夹心微滴阵列平台，我们已经证明了具有浓度和体积梯度的组合液滴阵列的高通量生成能力。此外，酶学研究的潜力已通过打印平台实施的量化纤维素反应得到验证。