Tuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.

中文翻译：

---

使用微流体声学拉曼平台实时监测活的分枝杆菌。

结核病（TB）仍然是全球范围内的主要死亡原因。脂类丰富，在表型上对抗生素具有耐受性，细菌对抗生素更具抵抗力，可能与复发和长期结核病治疗有关。我们提出了一种微流体系统，该系统在声学上捕获活的分枝杆菌，耻垢分枝杆菌，结核分枝杆菌的模型生物。然后，我们对捕获的耻垢分枝杆菌进行了长达八小时的波长调制拉曼光谱（WMRS）形式的光学分析，而且还存在异烟肼（INH）。暴露于INH的耻垢分枝杆菌的拉曼指纹与未施加压力的情况相比有很大变化。我们的工作提供了INH对这些分枝杆菌中脂质增加的影响的实时评估，这可能会使细胞对抗生素更具耐受性。