To explore the relationship between fruit developmental periods and the protein datasets of Alpinia oxyphylla, a label-free quantitative proteomic profiling analysis of the A. oxyphylla fruits sampled at the Earlystage, Metaphase and Advanced stage was carried out using the liquid chromatography combined with tandem mass spectrometry. A total of 19,219 peptide fragments and 4946 quantitative proteins were obtained. Annotation and enrichment analysis of these peptides and proteins were performed using the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases with bioinformatics software. The results showed that the differentially expressed proteins were mainly involved in metabolic and cellular processes, cellular component organization or biogenesis and response to the stimulus. Three significantly enriched metabolic pathways were non-alcoholic fatty liver disease, vibrio cholera infection and valine, leucine and isoleucine degradation. The significantly enriched proteins were NADH dehydrogenase, putative vacuolar proton translocation ATPase and putative acyltransferase component. The proteomic profiles of the fruit samples from the Advanced and the Metaphase stages differed little, while the difference in proteomic components between the Earlystage and the Advanced groups was significant. This study should lay the theoretical foundation for the effective utilization of A. oxyphylla germplasm resource in the treatment of human diseases.

中文翻译：

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无标签定量蛋白质组分析通过质谱鉴定潜在的活性成分，以发挥益智果实中药理作用

探索果发育时期，蛋白质数据集之间的关系益智的的无标记定量蛋白质组学分析分析一。氧化叶采用液相色谱-串联质谱法对早期，中期和晚期样品进行了分析。总共获得19,219个肽片段和4946个定量蛋白质。这些肽和蛋白质的注释和富集分析使用基因本体论（GO）和《京都议定书》的基因和基因组百科全书（KEGG）数据库以及生物信息学软件进行。结果表明，差异表达的蛋白质主要参与代谢和细胞过程，细胞成分的组织或生物发生和对刺激的反应。三种显着丰富的代谢途径是非酒精性脂肪肝疾病，霍乱弧菌感染和缬氨酸，亮氨酸和异亮氨酸降解。富含蛋白质的是NADH脱氢酶，假定的液泡质子易位ATP酶和假定的酰基转移酶成分。晚期阶段和中期阶段的水果样品的蛋白质组学特征差异不大，而早期阶段和高级阶段之间的蛋白质组学成分差异却很明显。该研究应为有效利用水产养殖奠定理论基础。一。氧叶种质资源治疗人类疾病。