CmFT homologous gene in muskmelon was obtained by homologous cloning, introducing CmFT gene by Agrobacterium-mediated transformation. The results of subcellular localization showed that CmFT protein was expressed in cytoplasm and nucleus. qRT-PCR results showed that the expression levels of AtLFY, AtFT, AtCO, AtFLC, AtSOC1 and AtAP1 were upregulated in the 35S::MeFTArabidopsis line. The CmFT gene was introduced into wild-type Arabidopsis by Agrobacterium-mediated transformation, and the growth status of T2 transgenic Arabidopsis thaliana and wild-type A. thaliana was observed. The results showed that wild-type Arabidopsis began to bolt on the 25th day after sowing, we can initially confirm that the FT gene of melon can promote the early flowering of melon in the growth and development of melon.

中文翻译：

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来自 Cucumis melo L 的 CmFT (FLOWERING LOCUS T) 的分子克隆和功能表征。

通过同源克隆获得甜瓜中的CmFT同源基因，通过农杆菌介导的转化引入CmFT基因。亚细胞定位结果表明CmFT蛋白在细胞质和细胞核中均有表达。qRT-PCR 结果显示 AtLFY、AtFT、AtCO、AtFLC、AtSOC1 和 AtAP1 的表达水平在 35S::MeFTArabidopsis 系中上调。通过农杆菌介导的转化将CmFT基因导入野生型拟南芥，观察T2转基因拟南芥和野生型拟南芥的生长情况。结果表明，野生型拟南芥在播种后第25天开始抽薹，初步证实瓜类FT基因在瓜类生长发育过程中可促进瓜类早花。