In the present study, the in vitro antifungal potential of seven Lamiaceae herbs against Fusarium oxysporum f. sp. cyclaminis were investigated. On the basis of the results, the in vivo antifungal effects of sage water extract on Fusarium wilt in cyclamen were evaluated, and the antifungal properties of the extract were identified. Among the seven herbs, sage water extract (0.5% and 2%, w/v) showed considerable suppression of F. oxysporum f. sp. cyclaminis in vitro. For the in vivo evaluation, cyclamen plants were treated with sage water extract (20%, w/v) two times and inoculated with F. oxysporum f. sp. cyclaminis. Six weeks after the inoculation, a lower disease incidence and disease severity indices were observed in roots and shoots of the treated plants. These effects could be attributed to reduced Fusarium populations in the plant roots because of the fungistatic and fungicidal effects of the sage extract. Consequently, the dry weights of the shoots and roots treated with the sage extract were higher than those of the control. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) was performed, and caffeic acid and rosmarinic acid were identified to be important metabolites in the sage water extract. In addition, the antifungal effects of these compounds against F. oxysporum f. sp. cyclaminis were confirmed using an in vitro test. Thus, it can be concluded that sage water extract can suppress Fusarium wilt in cyclamen and the two identified compounds play key roles in the antifungal properties of sage.

中文翻译：

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鼠尾草水提取物抑制仙客来枯萎病及抗真菌代谢产物鉴定

在本研究中，七种唇形科草药对尖孢镰刀菌的体外抗真菌潜力。sp. 仙客来进行了调查。在此结果的基础上，评价了鼠尾草水提取物对仙客来枯萎病的体内抗真菌作用，并鉴定了提取物的抗真菌特性。在七种草药中，鼠尾草水提取物（0.5% 和 2%，w/v）显示出对尖孢镰刀菌的显着抑制。sp. 仙客来体外。对于体内评价，仙客来植物用鼠尾草水提取物（20%，w/v）处理两次，并接种尖孢镰刀菌。sp. 仙客来。接种六周后，在处理植物的根和芽中观察到较低的发病率和疾病严重程度指数。由于鼠尾草提取物的抑菌和杀真菌作用，这些影响可能归因于植物根部镰刀菌种群的减少。因此，用鼠尾草提取物处理的芽和根的干重高于对照。进行了超高效液相色谱-串联质谱 (UPLC-MS)，确定咖啡酸和迷迭香酸是鼠尾草水提取物中的重要代谢物。此外，这些化合物对尖孢镰刀菌的抗真菌作用。sp. 仙客来经体外试验证实。因此，可以得出结论，鼠尾草水提取物可以抑制仙客来的枯萎病，并且这两种已鉴定的化合物在鼠尾草的抗真菌特性中起着关键作用。因此，用鼠尾草提取物处理的芽和根的干重高于对照。进行了超高效液相色谱-串联质谱 (UPLC-MS)，确定咖啡酸和迷迭香酸是鼠尾草水提取物中的重要代谢物。此外，这些化合物对尖孢镰刀菌的抗真菌作用。sp. 仙客来经体外试验证实。因此，可以得出结论，鼠尾草水提取物可以抑制仙客来的枯萎病，并且这两种已鉴定的化合物在鼠尾草的抗真菌特性中起着关键作用。因此，用鼠尾草提取物处理的芽和根的干重高于对照。进行了超高效液相色谱-串联质谱 (UPLC-MS)，确定咖啡酸和迷迭香酸是鼠尾草水提取物中的重要代谢物。此外，这些化合物对尖孢镰刀菌的抗真菌作用。sp. 仙客来经体外试验证实。因此，可以得出结论，鼠尾草水提取物可以抑制仙客来的枯萎病，并且这两种已鉴定的化合物在鼠尾草的抗真菌特性中起着关键作用。咖啡酸和迷迭香酸被鉴定为鼠尾草水提取物中的重要代谢物。此外，这些化合物对尖孢镰刀菌的抗真菌作用。sp. 仙客来经体外试验证实。因此，可以得出结论，鼠尾草水提取物可以抑制仙客来的枯萎病，并且这两种已鉴定的化合物在鼠尾草的抗真菌特性中起着关键作用。咖啡酸和迷迭香酸被鉴定为鼠尾草水提取物中的重要代谢物。此外，这些化合物对尖孢镰刀菌的抗真菌作用。sp. 仙客来经体外试验证实。因此，可以得出结论，鼠尾草水提取物可以抑制仙客来的枯萎病，并且这两种已鉴定的化合物在鼠尾草的抗真菌特性中起着关键作用。