This study determined the effect of orexin B (OXB) on the porcine endometrial transcriptome during the embryo attachment phase. Microarray analyses of gene ontology (GO), biological pathways, networks and differentially expressed genes (DEG) were performed. Orexin B influenced the expression of 887 genes (fold change > 1.2; p < .05): 620 genes were up‐regulated, and 267 were down‐regulated. The analysis of the relationship between DEG revealed that OXB interacts with genes linked with processes such as cell hormone binding, regulation of hormone levels, lipid transport, steroid metabolic processes, the apoptotic signalling pathway and the acute inflammatory response, which are pivotal for reproductive success. Orexin B played a bivalent role in the early‐pregnant uterus by limiting the pregnancy outcome, promoting embryo development, suppressing the immune system and, consequently, preventing embryo rejection. These findings suggest that OXB could be responsible for the proper course of gestation by adapting litter size to the metabolic status of the maternal organism.

中文翻译：

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Orexin B从植入早期开始影响体外培养的猪子宫内膜外植体的转录组

这项研究确定了orexin B（OXB）在胚胎附着阶段对猪子宫内膜转录组的影响。进行了基因本体（GO），生物学途径，网络和差异表达基因（DEG）的微阵列分析。Orexin B影响了887个基因的表达（倍数变化> 1.2；p <.05）：620个基因被上调，而267个基因被下调。对DEG之间关系的分析表明，OXB与与细胞激素结合，激素水平调节，脂质转运，类固醇代谢过程，细胞凋亡信号通路和急性炎症反应等过程相关的基因相互作用，这对于生殖成功至关重要。食欲素B通过限制妊娠结局，促进胚胎发育，抑制免疫系统并因此防止胚胎排斥，在早孕子宫中起着双价作用。这些发现表明，OXB可能通过使产仔数适应母体生物的代谢状态来负责适当的妊娠过程。