In this study, we aimed to characterize lactic acid bacteria strains derived from infants' feces, to evaluate the probiotic potential and explore the wide-spectrum antimicrobial activity. Of 800 isolates, 20 inhibited the growth of enterotoxigenic Escherichia coli K88 and Salmonella enterica ATCC 13076. On the basis of 16S rRNA sequence analysis, the 20 isolates were assigned to Lactobacillus casei (7), Lactobacillus paracasei (2), Lactobacillus plantarum (4), Lactobacillus rhamnosus (2), Enterococcus avium (3), Enterococcus faecium (1), and Enterococcus lactis (1) species. In addition, 12 strains with high antimicrobial activity were investigated for the presence of probiotic properties such as physiological-biochemical characteristics, antimicrobial susceptibility, hemolytic activity, hydrophobicity, and aggregation activity. Wide-spectrum antimicrobial activity analysis revealed that approximately all tested strains inhibited the ten pathogens, and four strains (ZX221, ZX633, ZX3131, and ZX3875) had good probiotic properties and survived after being exposed to simulated gastrointestinal tract conditions. Moreover, we investigated the influence of pH on the wide-spectrum antimicrobial activity and found that four strains inhibited most pathogens at pH 4.5 and pH 5, whereas only ZX633 had an inhibitory effect on Bacillus subtilis ATCC 6633 and Micrococcus luteus ATCC 4698 at pH 5.5. Overall, Lact. casei ZX633 had wide-spectrum antimicrobial activity and could be considered a potential probiotic.

中文翻译：

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从婴儿粪便中分离出的乳酸菌的益生菌潜力和广谱抗菌活性。

在这项研究中，我们旨在表征源自婴儿粪便的乳酸菌菌株，以评估益生菌的潜力并探索广谱抗菌活性。在800株菌株中，有20株抑制了产肠毒素的大肠杆菌K88和小肠沙门氏菌ATCC 13076的生长。根据16S rRNA序列分析，将这20株菌株分别命名为干酪乳杆菌（7），副干酪乳杆菌（2），植物乳杆菌（4）。 ），鼠李糖乳杆菌（2），鸟肠球菌（3），粪肠球菌（1）和乳酸肠球菌（1）种。另外，对12株具有高抗菌活性的菌株进行了益生菌特性研究，例如生理生化特性，抗菌药敏性，溶血活性，疏水性和聚集活性。广谱抗菌活性分析表明，几乎所有测试菌株均能抑制10种病原体，其中4种菌株（ZX221，ZX633，ZX3131和ZX3875）具有良好的益生菌性质，并在暴露于模拟胃肠道条件下可以存活。此外，我们研究了pH对广谱抗菌活性的影响，发现四种菌株在pH 4.5和pH 5时能抑制大多数病原体，而只有ZX633在pH 5.5时对枯草芽孢杆菌ATCC 6633和黄球微球菌ATCC 4698有抑制作用。 。总体而言，Lact。酪蛋白ZX633具有广谱抗菌活性，可以认为是潜在的益生菌。（ZX3875）和ZX3875）具有良好的益生菌性质，并在模拟胃肠道条件下存活。此外，我们研究了pH对广谱抗菌活性的影响，发现四种菌株在pH 4.5和pH 5时能抑制大多数病原体，而只有ZX633在pH 5.5时对枯草芽孢杆菌ATCC 6633和黄球微球菌ATCC 4698有抑制作用。 。总体而言，Lact。酪蛋白ZX633具有广谱抗菌活性，可以认为是潜在的益生菌。（ZX3875）和ZX3875）具有良好的益生菌性质，并在模拟胃肠道条件下存活。此外，我们研究了pH对广谱抗菌活性的影响，发现四种菌株在pH 4.5和pH 5时能抑制大多数病原体，而只有ZX633在pH 5.5时对枯草芽孢杆菌ATCC 6633和黄球微球菌ATCC 4698有抑制作用。 。总体而言，Lact。酪蛋白ZX633具有广谱抗菌活性，可以认为是潜在的益生菌。而只有ZX633在pH 5.5时对枯草芽孢杆菌ATCC 6633和黄褐微球菌ATCC 4698具有抑制作用。总体而言，Lact。酪蛋白ZX633具有广谱抗菌活性，可以认为是潜在的益生菌。而只有ZX633在pH 5.5时对枯草芽孢杆菌ATCC 6633和黄褐微球菌ATCC 4698具有抑制作用。总体而言，Lact。酪蛋白ZX633具有广谱抗菌活性，可以认为是潜在的益生菌。