This study investigates the effect of polydatin on the neurological function of cerebral hemorrhage rats and on the Nrf2 pathway of the endogenous antioxidant system in tissues around cerebral hematoma. Further, the study also aims to provide solid insights for clinical diagnosis and treatment. A total of 54 SPF grade male Wistar rats were divided into three groups: the sham group, model group, and polydatin group. Various parameters such as neurological deficit score, brain water content, pathological morphology, oxidative stress index content, Nrf2, NQO1, HO-1 mRNA expression, and the expression of HO-1, Nrf2, and kelch-like epichlorohydrin-1 (Keap1) protein were observed. Compared with the sham group, the mNSS score and brain water content of rats in the model group increased significantly after dosing (P < 0.05). When compared with the model group, the mNSS score and brain water content of rats in the polydatin group decreased significantly after dosing (P < 0.05). Compared with the other group, the serum NSE content of rats in the polydatin group decreased (P < 0.05). An increase was observed in the contents of NO, SOD, MDA, GSSG, and GSH in the brain tissue of rats in the model group when compared with the sham group. Compared with the model group, the contents of NO and MDA in the brain tissue of rats in the polydatin group decreased, while the contents of SOD, GSSG, and GSH increased (P < 0.05). The relative expressions of Nrf2, NQO1, and HO-1mRNA in the brain tissue of rats in the polydatin group was relatively high compared with both groups (P < 0.05). Polydatin can improve the neurological function of ICH rats and reduce the oxidative stress response by regulating the Nrf2-ARE pathway and downstream gene expression. This study preliminarily discussed the relevant mechanism of polydatin in the treatment of ICH rats, thus providing a theoretical reference to ICH treatment.

中文翻译：

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虎杖苷对脑出血时神经功能及 Nrf2 通路的影响。

本研究探讨虎杖苷对脑出血大鼠神经功能及脑血肿周围组织内源性抗氧化系统Nrf2通路的影响。此外，该研究还旨在为临床诊断和治疗提供可靠的见解。将54只SPF级雄性Wistar大鼠分为3组：假手术组、模型组和虎杖苷组。神经功能缺损评分、脑含水量、病理形态学、氧化应激指标含量、Nrf2、NQO1、HO-1 mRNA表达、HO-1、Nrf2、kelch-like epichlorohydrin-1(Keap1)表达等多种参数观察到蛋白质。与假手术组比较，模型组大鼠给药后mNSS评分和脑含水量均显着升高（P < 0.05)。与模型组比较，虎杖苷组大鼠给药后 mNSS 评分和脑水含量均显着降低（P  < 0.05）。与其他各组比较，虎杖苷组大鼠血清 NSE 含量降低（P  < 0.05）。模型组大鼠脑组织NO、SOD、MDA、GSSG、GSH含量较假手术组升高。与模型组比较，虎杖苷组大鼠脑组织中 NO、MDA 含量降低，SOD、GSSG、GSH 含量升高（P  < 0.05）。虎杖苷组大鼠脑组织中Nrf2、NQO1、HO-1mRNA的相对表达量较两组均较高（P  < 0.05）。虎杖苷可通过调控 Nrf2-ARE 通路及下游基因表达，改善 ICH 大鼠的神经功能，减轻氧化应激反应。本研究初步探讨虎杖苷治疗脑出血大鼠的相关机制，为脑出血治疗提供理论参考。