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Production, partial purification and characterization of a proteoglycan bioemulsifier from an oleaginous yeast.
Bioprocess and Biosystems Engineering ( IF 3.8 ) Pub Date : 2020-05-14 , DOI: 10.1007/s00449-020-02361-1
Moumita Bhaumik 1 , Gunaseelan Dhanarajan 1 , Jayita Chopra 1 , RaviRanjan Kumar 1 , Chinmay Hazra 1 , Ramkrishna Sen 1
Affiliation  

In this study, Meyerozyma caribbica, an indigenously isolated oleaginous yeast, produced in media containing glucose a bioemulsifier that was partially characterized as a proteoglycan based on preliminary analysis. Optimization of carbon:nitrogen (C:N) ratio revealed 30:1 as the suitable ratio for enhanced production. Apart from higher emulsification activity (E24: 70-80%), this molecule showed strong emulsion stability over a wide range of pH (2.0-9.0), salinity (0.05%-10%, w/v) and temperature (- 80 °C to + 50 °C). The current study emphasizes on the determination of critical media parameters for improved and stable bioemulsifier production coupled with partial characterization and identification of the molecule. Thus, a proteoglycan-based bioemulsifier with such a stable emulsifying property can serve as a versatile and potential component in food, cosmetics and pharmaceutical formulations.

中文翻译:

从油质酵母生产,部分纯化和鉴定蛋白聚糖生物乳化剂。

在这项研究中,Carybica Meyerozyma caribbica是一种原产地分离的油质酵母,在含有葡萄糖的培养基中产生了一种生物乳化剂,根据初步分析,该乳化剂的部分特征是蛋白聚糖。碳:氮(C:N)比的优化表明30:1是提高产量的合适比例。除了较高的乳化活性(E24:70-80%)外,该分子在广泛的pH值(2.0-9.0),盐度(0.05%-10%,w / v)和温度(-80° C至+ 50°C)。当前的研究着重于确定关键介质参数,以改善和稳定生产生物乳化剂,同时对分子进行部分表征和鉴定。从而,
更新日期:2020-05-14
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