Infectious bursal disease virus (IBDV), which infects young chickens, is one of the most important pathogens that harm the poultry industry. Evaluation of the immune status of birds before and after vaccination is of great importance for controlling the disease caused by this virus. Therefore, the development of low-cost and easy-to-manufacture test systems for IBDV antibody detection remains an urgent issue. In this study, three expression systems (bacteria, yeast, and human cells) were used to produce recombinant VP3 protein of IBDV. VP3 is a group-specific antigen and hence may be a good candidate for use in diagnostic tests. Comparison of the antigenic properties of the obtained polypeptides showed that the titres of antibodies raised in chickens against bacteria- or human-cell-derived recombinant VP3 were high, whereas the antibody level against yeast-derived recombinant VP3 was low. The results of an enzyme-linked immunosorbent assay (ELISA) of sera from IBDV-infected chickens demonstrated that the recombinant VP3 produced in E. coli would be the best choice for use in test systems.

中文翻译：

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在三种不同表达系统中产生传染性法氏囊病病毒重组VP3蛋白，对获得的多肽进行抗原分析并开发ELISA测试。

传染性法氏囊病病毒（IBDV）感染幼鸡，是危害家禽业的最重要病原体之一。评估疫苗接种前后禽类的免疫状态对于控制该病毒引起的疾病具有重要意义。因此，开发低成本且易于制造的IBDV抗体检测系统仍然是一个紧迫的问题。在本研究中，使用三种表达系统（细菌、酵母和人类细胞）来生产IBDV的重组VP3蛋白。VP3 是一种群体特异性抗原，因此可能是用于诊断测试的良好候选者。所获得的多肽的抗原特性的比较表明，在鸡中产生的针对细菌或人细胞来源的重组VP3的抗体滴度较高，而针对酵母来源的重组VP3的抗体水平较低。对感染 IBDV 的鸡血清进行的酶联免疫吸附测定 (ELISA) 结果表明，大肠杆菌中产生的重组 VP3 将是用于测试系统的最佳选择。