Abstract Phloroglucinol (PG) is an important chemical that has been produced in engineered E. coli. Low yield and high cost are the bottlenecks that limit the industrialisation and popularisation of PG biosynthesis. In this study, a new expression system (pNEW vector) was developed for PG biosynthesis by using inexpensive p-isopropylbenzoate (cumate) as the inducer, and the copy number control element (rop) was added to optimise the original pNEW vector, which not only promoted the PG yield, but also reduced the leakage expression. Compared to traditional IPTG inducible expression system, 2-fold enhancement of PG yield was obtained with optimised expression system and the cost of inducer was reduced to 1/9. These results provide technical support for the biosynthesis of PG and reference for the selection of induced expression systems for other chemical biosynthesis.

中文翻译：

---

提高间苯三酚产量的新表达系统

摘要 间苯三酚 (PG) 是一种重要的化学物质，已在工程大肠杆菌中产生。低收率和高成本是限制PG生物合成产业化和推广的瓶颈。本研究以廉价的苯甲酸对异丙酯（cumate）为诱导剂，开发了一种新的PG生物合成表达系统（pNEW载体），并加入拷贝数控制元件（rop）优化原有pNEW载体，不仅提高了PG产量，而且还减少了泄漏表达。与传统IPTG诱导型表达系统相比，优化后的表达系统使PG产量提高2倍，诱导剂成本降低至1/9。