ABSTRACT

Background: Oral mucositis (OM) is a common side effect of conditioning therapy implemented before hematopoietic stem cell transplantation (HSCT). The role of oral microbiome in OM is not fully elucidated.

Objective: To determine oral microbiome profile changes post-conditioning in HSCT patients who developed moderate OM, or mild to no OM.

Design: Patient groups were: Muc0-1 with OM-score = 0–1 (43 paired samples) and Muc2 with WHO OM-score = 2 (36 paired samples). Bacterial DNA was isolated from oral samples (saliva, swabs of buccal mucosa, tongue, and supragingival plaque) at pre-conditioning (T₀), post-conditioning mucositis onset (T_Muc), and one-year post-conditioning (T_Year). 16S-rRNA gene next-generation sequencing was used to determine the relative abundance (RA) of >700 oral species. Alpha-diversity, beta-diversity and linear discriminant analyses (LDA) were performed Muc2 versus Muc0-1.

Results: Muc2 oral microbiome alpha- and beta-diversity differed between T₀ and T_Muc. Muc2 alpha-diversity and Muc0-1 beta-diversity did not differ between T₀ and T_Year. T₀ to T_Muc LDA scores were significant in Muc2 for Gammaproteobacteria. For Muc2 patients, the average RA decreased for Haemophilus parainfluenza, a species known as mucosal surfaces protector, but increased for Escherichia-Shigella genera.

Conclusions: Post-conditioning OM might contribute to long-term oral microbiome changes affecting Gammaproteobacteria, in HSCT patients.

摘要

背景：口腔粘膜炎（OM）是造血干细胞移植（HSCT）前进行的调理治疗的常见副作用。口腔微生物组在 OM 中的作用尚未完全阐明。

目的：确定出现中度 OM 或轻度至无 OM 的 HSCT 患者在调理后口腔微生物组特征的变化。

设计：患者组为：Muc0-1，OM 评分 = 0–1（43 个配对样本），Muc2，WHO OM 评分 = 2（36 个配对样本）。在预处理 (T ₀ )、处理后粘膜炎发作 (T _Muc ) 和处理一年后 (T _Year ) 时从口腔样本（唾液、颊粘膜拭子、舌头和龈上菌斑）中分离细菌 DNA ）。16S-rRNA基因新一代测序用于确定 > 700 个口腔物种的相对丰度 (RA)。对 Muc2与Muc0-1进行α-多样性、β-多样性和线性判别分析 (LDA) 。

结果： T ₀和 T _Muc之间Muc2 口腔微生物组α和β多样性不同。Muc2 α多样性和 Muc0-1 β多样性在 T ₀和 T _Year之间没有差异。T ₀至 T _Muc LDA 评分在 Muc2 中对于Gammaproteobacteria具有显着性。对于 Muc2 患者，副流感嗜血杆菌（一种被称为粘膜表面保护者的物种）的平均 RA 降低，但埃希氏菌-志贺菌属的平均 RA 增加。

结论：后处理 OM 可能会导致HSCT 患者口腔微生物群的长期变化，从而影响γ 变形菌。