Aims: In this study, we prepared spirulina polysaccharides into spirulina polysaccharide-loaded nanoemulsions (SPS-NEs), and determined the antitumor effect of SPS-NEs, when combined with paclitaxel (PTX). Methods: SPS-NEs were prepared by a phase transformation method. The Characterization and stability of SPS-NEs was measured. The antitumor effect of SPS-NEs combined with PTX was determined by S180 cells or RAW 264.7 macrophages and S180 tumor-bearing mice. Results: SPS-NEs were spherical and stable, the particle size of SPS-NEs was 84.6 ± 3.31 nm, PDI =0.235 ± 0.02. PTX + SPS-NEs exhibited a much greater toxicity against RAW 264.7 cells than PTX. PTX + SPS-NEs increased the release of NO, IL-6 and TNF-α, and the expression of p-p65 NF-κB, p-I-κB, TLR4. In addition, PTX + SPS-NEs significantly inhibited tumor growth by 72.82% and increased the secretion of serum IL-2, TNF-α and IFN-γ. Conclusions: SPS-NEs can regulate immunity through TLR4/NF-κB signaling pathways, which enhances the anti-tumor effect of PTX.

目的：在这项研究中，我们将螺旋藻多糖制备为负载螺旋藻多糖的纳米乳剂（SPS-NEs），并确定了与紫杉醇（PTX）结合使用时SPS-NEs的抗肿瘤作用。方法：采用相变法制备SPS-NEs。测量了SPS-NE的特性和稳定性。通过S180细胞或RAW 264.7巨噬细胞和S180荷瘤小鼠确定SPS-NEs与PTX结合的抗肿瘤作用。结果：SPS-NEs为球形且稳定，SPS-NEs的粒径为84.6±3.31 nm，PDI = 0.235±0.02。PTX + SPS-NEs对RAW 264.7细胞的毒性比PTX大得多。PTX + SPS-NEs增加NO，IL-6和TNF-α的释放以及p-p65NF-κB，pI-κB，TLR4的表达。此外，PTX + SPS-NEs显着抑制肿瘤生长达72.82％，并增加了血清IL-2，TNF-α和IFN-γ的分泌。结论： SPS-NEs可通过TLR4 /NF-κB信号通路调节免疫力，增强PTX的抗肿瘤作用。