Here, the retinal targeting SA-g-AA coated multilamellar liposomes carrier synthesized to deliver the bioactive agents into the retinal region of the eye. The multilayered targeting macromolecules of liposomes prepared using a layer-by-layer assembly. The curcumin (CUR) and Rhodamine B (RhB) dyes loaded in a multilamellar vesicle (MLV) were synthesized by the Lipid film hydration method. The Sodium alginate grafted acrylic acid (SA-g-AA) conjugated with Riboflavin (RB) was coated over MLV by O/W emulsion method followed by ionotropic gelation. FT-IR and 1H NMR spectroscopy techniques used to analyze the structural features of the MLV-SA-g-AA-RB. The results of DLS and TEM revealed that the carrier could be of uniform spheres, with a low polydispersity index, and outstanding performance in phrases of dye encapsulation and extended-release ability. An MTT assay investigated cell viability against Fibroblast WS1, and human embryonic stem cells-derived Retinal Pigment Epithelial cells (hESC-RPE) implied that the carrier is of excellent biocompatibility. Retina targeting nature of the system confirmed via cellular uptake results revealed that the increases the dye concentration in the cells. Overall, the outcomes suggested that carriers could lead to the improvement of a feasible two photoreceptors targeting drug carriers, and it has the potential to deliver the multidrug in the retinal region of the eye.

中文翻译：

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视网膜光感受器靶向 SA-g-AA 涂层多层脂质体载体系统，用于细胞毒性和细胞摄取评估

在这里，合成了视网膜靶向 SA-g-AA 涂层的多层脂质体载体，以将生物活性剂输送到眼睛的视网膜区域。使用逐层组装制备的脂质体的多层靶向大分子。通过脂质膜水合法合成负载在多层囊泡 (MLV) 中的姜黄素 (CUR) 和罗丹明 B (RhB) 染料。将海藻酸钠接枝丙烯酸 (SA-g-AA) 与核黄素 (RB) 结合后通过 O/W 乳液法涂覆在 MLV 上，然后进行离子凝胶化。FT-IR 和 1H NMR 光谱技术用于分析 MLV-SA-g-AA-RB 的结构特征。DLS和TEM结果表明，该载体呈均匀的球形，多分散指数低，在染料包封和缓释性能方面表现突出。MTT 测定研究了针对成纤维细胞 WS1 的细胞活力，人类胚胎干细胞衍生的视网膜色素上皮细胞 (hESC-RPE) 表明该载体具有出色的生物相容性。通过细胞摄取结果证实的系统的视网膜靶向性质表明，增加了细胞中的染料浓度。总体而言，结果表明，载体可能会导致改进一种可行的针对药物载体的两个光感受器，并且它有可能在眼睛的视网膜区域递送多药。通过细胞摄取结果证实的系统的视网膜靶向性质表明，增加了细胞中的染料浓度。总体而言，结果表明，载体可能会导致改进一种可行的针对药物载体的两个光感受器，并且它有可能在眼睛的视网膜区域递送多药。通过细胞摄取结果证实的系统的视网膜靶向性质表明，增加了细胞中的染料浓度。总体而言，结果表明，载体可能会导致改进一种可行的针对药物载体的两个光感受器，并且它有可能在眼睛的视网膜区域递送多药。