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Protective effects of extracts of lichen Dirinaria consimilis (Stirton) D.D. Awasthi in bifenthrin- and diazinon-induced oxidative stress in rat erythrocytes in vitro
Drug and Chemical Toxicology ( IF 2.6 ) Pub Date : 2020-05-12 , DOI: 10.1080/01480545.2020.1762632
Vinay Bharadwaj Tatipamula 1, 2 , Biljana Kukavica 3
Affiliation  

Abstract

The intoxication of insecticides such as bifenthrin and diazinon has been reported to generate free radicals, and thereby alter the antioxidant defense system in erythrocytes. The present study is aimed to investigate the protective effects of acetone (DA) and methanolic (DM) extracts of lichen Dirinaria consimilis against bifenthrin and diazinon toxicity in rats’ erythrocytes in vitro. Rats’ erythrocytes were exposed to bifenthrin and diazinon, individually and also in combination with DA or DM at 1 ppm for 3 h at 37 ˚C. By using spectrophotometric methods, all the samples were estimated for changes in hemoglobin (Hb) concentration, malondialdehyde (MDA) levels, and enzyme [Superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferases (GST)] activities. The outcomes showed that both the insecticides were highly toxic to rats’ erythrocytes. Among all groups, both the insecticides and DA exposed groups showed very low levels of MDA content, and GST activity in rats’ erythrocytes, when compared to the control. Besides, DA groups pretreated with both insecticides showed significant improvement of total Hb concentration, SOD, and CAT activities, with respect to the control. Hence, the present results indicate that the extracts of D. consimilis act as an antioxidant agent that reduces oxidative stress burden in insecticides toxicity.



中文翻译:

地衣 Dirinaria consimilis (Stirton) DD Awasthi 提取物对联苯菊酯和二嗪农诱导的大鼠红细胞氧化应激的体外保护作用

摘要

据报道,杀虫剂如联苯菊酯和二嗪农的中毒会产生自由基,从而改变红细胞中的抗氧化防御系统。本研究旨在探讨地衣的丙酮(DA)和甲醇(DM)提取物大鼠红细胞中联苯菊酯和二嗪农毒性的保护作用。. 将大鼠的红细胞单独暴露于联苯菊酯和二嗪农,也可与 1 ppm 的 DA 或 DM 在 37°C 下组合 3 小时。通过使用分光光度法,评估所有样品的血红蛋白 (Hb) 浓度、丙二醛 (MDA) 水平和酶 [超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT) 和谷胱甘肽 S-转移酶 (GST)] 活性的变化。结果表明,这两种杀虫剂对大鼠的红细胞都有剧毒。在所有组中,与对照组相比,杀虫剂组和 DA 暴露组的 MDA 含量和大鼠红细胞中的 GST 活性都非常低。此外,与对照相比,用两种杀虫剂预处理的 DA 组显示总 Hb 浓度、SOD 和 CAT 活性显着改善。因此,D. consimilis作为一种抗氧化剂,可减少杀虫剂毒性中的氧化应激负担。

更新日期:2020-05-12
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