ABSTRACT Aim The study explored the beneficial effects of repeated episodes of whole body hypoxic perconditioning (WHPC) on preeclampsia (PE)-like symptoms in rats. Material and Methods PE was induced by administration of L-NAME (75 mg/kg) and WHPC was performed by exposing rats to low O2 (8%) and normal O2 of 10 min each in four alternate cycles. Results L-NAME induced PE like symptoms in rats along with a decrease in the cystathionine-β-synthase (CBS) activity in the placental tissue, plasma levels of H2S and NO metabolites in pregnant rats. Two (GD9, GD14) and three episodes (GD9, GD14 and GD18) of WHPC improved PE-like symptoms with an increase in CBS activity and H2S levels. CBS inhibitor, amino-oxyacetic acid abolished the beneficial effects of three episodes of WHPC; while H2S donor, 4-methylbenzenecarbothioamide, 4-MBC attenuated PE-like symptoms. Conclusion WHPC attenuates L-NAME-induced PE-like symptoms due to increase in CBS activity and H2S-production.

中文翻译：

---

探索反复发作的全身缺氧预处理对先兆子痫大鼠模型的有益作用和可能机制

摘要 目的 本研究探讨反复发作的全身缺氧预处理 (WHPC) 对大鼠先兆子痫 (PE) 样症状的有益影响。材料和方法 PE 是通过施用 L-NAME (75 mg/kg) 诱导的，WHPC 是通过将大鼠暴露于低 O2 (8%) 和正常 O2 的四个交替循环中，每一个 10 分钟。结果 L-NAME 在大鼠中诱发 PE 样症状，同时胎盘组织中的胱硫醚-β-合酶 (CBS) 活性、妊娠大鼠的血浆 H2S 和 NO 代谢物水平降低。WHPC 的两集（GD9、GD14）和三集（GD9、GD14 和 GD18）通过增加 CBS 活性和 H2S 水平改善了 PE 样症状。CBS 抑制剂氨基-羟基乙酸消除了三期 WHPC 的有益作用；而 H2S 供体，4-甲基苯硫代酰胺，4-MBC 可减轻 PE 样症状。结论 由于 CBS 活性和 H2S 产生增加，WHPC 可减轻 L-NAME 诱导的 PE 样症状。