The serum or plasma microRNA (miRNA) molecules have been suggested as diagnostic and prognostic biomarkers, in various pathological conditions. However, these molecules are also found in different serum fractions, such as exosomes and Argonaute (Ago) protein complexes. Ago1 is the predominant Ago protein expressed in heart tissue. The objective of the study was to examine the hypothesis that Ago1‐associated miRNAs may be more relevant to cardiac disease and heart failure compared with the serum. In total, 84 miRNA molecules were screened for their expression in the whole serum, exosomes and Ago1, and Ago2 complexes. Ago1‐bound miR‐222‐3p, miR‐497‐5p and miR‐21‐5p were significantly higher, and let‐7a‐5p was significantly lower in HF patients compared with healthy controls, whereas no such difference was observed for those markers in the serum samples among the groups. A combination of these 4 miRNAs into an Ago1‐HF score provided a ROC curve with an AUC of 1, demonstrating clear discrimination between heart failure patients and healthy individuals. Ago1 fraction might be a better and more specific platform for identifying HF‐related miRNAs compared with the whole serum.

中文翻译：

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microRNA在血清组分中的差异表达以及Argonaute 1 microRNA与心力衰竭的关系。

血清或血浆microRNA（miRNA）分子已被建议在各种病理条件下作为诊断和预后生物标志物。但是，这些分子也存在于不同的血清组分中，例如外泌体和Argonaute（Ago）蛋白复合物。Ago1是在心脏组织中表达的主要Ago蛋白。这项研究的目的是检验与血清相比，与Ago1相关的miRNA与心脏病和心力衰竭更为相关的假设。总共筛选了84个miRNA分子在整个血清，外泌体，Ago1和Ago2复合物中的表达。与健康对照组相比，HF患者的Ago1结合miR‐222‐3p，miR‐497‐5p和miR‐21‐5p显着升高，let‐7a‐5p显着降低，而各组之间血清样品中的这些标记物没有观察到这种差异。将这4个miRNA组合为Ago1-HF评分后，ROC曲线的AUC为1，这表明心力衰竭患者与健康个体之间存在明显的区别。与整个血清相比，Ago1馏分可能是鉴定HF相关miRNA的更好，更特异性的平台。