Wings are an important flight organ of insects and their morphogenesis depends on a series of cell-cell and cell-extracellular matrix interactions. Integrin as a transmembrane protein receptor mediates cell-cell adhesion, cell-extracellular matrix interactions and signal transduction. In the present study, we characterized an integrin gene that encodes Integrinβ-PS protein in Locusta migratoria. LmIntegrinβ-PS is highly expressed in the wing pads and the middle stages of fifth instar nymphs. Immunohistochemical analysis revealed that the LmIntegrinβ-PS protein was localized at the cell base of the two layers of wings. After suppression of LmIntegrinβ-PS by RNA interference, the wing pads or wings were unable to form normally, with a blister wing appearance during nymph to nymph transition and nymph to adult transition. We further found that the dorsal and ventral epidermis of the wings after dsLmIntegrinβ-PS injection were improperly connected and formed huge cavities revealed by hematoxylin and eosin staining. Furthermore, the morphology and structure of the wing cuticle was significantly disturbed which affected the stable arrangement and attachments of the wing epidermis. Moreover, the expression of related cell adhesion genes was significantly decreased in LmIntegrinβ-PS-suppressed L. migratoria, suggesting that LmIntegrinβ-PS is required for the morphogenesis and development of wings during molting by stabilizing cell adhesion and maintaining the cytoskeleton of these cells. This article is protected by copyright. All rights reserved.

中文翻译：

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LmIntegrinβ-PS 是飞蝗翅膀形态发生和发育所必需的

翅膀是昆虫重要的飞行器官，其形态发生依赖于一系列细胞-细胞和细胞-细胞外基质的相互作用。整合素作为跨膜蛋白受体介导细胞-细胞粘附、细胞-细胞外基质相互作用和信号转导。在本研究中，我们表征了一种在 Locusta migratoria 中编码 Integrinβ-PS 蛋白的整合素基因。LmIntegrinβ-PS 在五龄若虫的翅垫和中期高度表达。免疫组织化学分析表明，LmIntegrinβ-PS 蛋白定位于两层翅膀的细胞基部。通过RNA干扰抑制LmIntegrinβ-PS后，翼垫或翅膀无法正常形成，在若虫向若虫过渡和若虫向成虫过渡期间出现水泡翅膀。我们进一步发现，注射 dsLmIntegrinβ-PS 后翅膀的背侧和腹侧表皮连接不当，形成了苏木精和伊红染色显示的巨大空腔。此外，翼表皮的形态和结构受到显着干扰，影响翼表皮的稳定排列和附着。此外，LmIntegrinβ-PS 抑制的 L. migratoria 中相关细胞粘附基因的表达显着降低，表明 LmIntegrinβ-PS 是蜕皮过程中翅膀形态发生和发育所必需的，通过稳定细胞粘附和维持这些细胞的细胞骨架。本文受版权保护。版权所有。