Canine pyometra is a common inflammatory disease of uterus in sexually mature bitches caused by secondary bacterial infection, leading to change in plasma proteins associated with the innate immune system. Proteomic investigation is increasingly being applied to canine diseases in order to identify and quantify significant changes in the plasma proteome. The aim of the study was to assess and quantify changes in plasma proteome profiles of healthy and pyometra affected bitches using a TMT-based high-resolution quantitative proteomic approach. As a result, 22 proteins were significantly down-regulated including transthyretin, antithrombin III, retinol-binding protein, vitamin D binding protein, paraoxonase 1, and kallikrein, while 16 were significantly up-regulated including haptoglobin light chain, alpha-1-acid glycoprotein, C-reactive protein precursor, and lipopolysaccharide-binding protein in dogs with pyometra. Pathway analysis indicated that acute inflammatory response, regulation of body fluid levels, protein activation cascade, the humoral immune response, and phagocytosis were affected in pyometra. Validation of biological relevance of the proteomic study was evident with significant increases in the concentrations of haptoglobin, C-reactive protein, alpha 1 acid glycoprotein, and ceruloplasmin by immunoassay. Pyometra in bitches was shown to stimulate an increase in host defence system proteins in response to inflammatory disease including the acute phase proteins. SIGNIFICANCE: The label-based high-resolution quantitative proteomics analysis and bioinformatic approach used in this study provide insight into the complex pathophysiology of inflammation associated with pyometra revealing proteins with biomarker potential. Early diagnosis and therapeutic intervention may prevent severe complications associated with advancing sepsis in dogs with pyometra. Therefore the identification of diagnostic biomarkers that, after clinical validation may be used in veterinary practice and protein relevant to pathways responding to disease are important findings of the study. Data are available via ProteomeXchange with identifier PXD015951.

中文翻译：

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犬pyometra中的血浆蛋白质组和急性期蛋白反应。

犬脓毒症是继发性细菌感染引起的性成熟雌性中常见的子宫炎性疾病，导致与先天免疫系统相关的血浆蛋白发生变化。蛋白质组学研究正越来越多地应用于犬类疾病，以鉴定和量化血浆蛋白质组学的重大变化。该研究的目的是使用基于TMT的高分辨率定量蛋白质组学方法评估和量化健康和受pyometra影响的母犬血浆蛋白质组谱的变化。结果，包括运甲状腺素蛋白，抗凝血酶III，视黄醇结合蛋白，维生素D结合蛋白，对氧磷酶1和激肽释放酶的22种蛋白被显着下调，而包括触珠蛋白轻链，α-1-酸的16种蛋白被显着上调。糖蛋白 脓毒症犬的C反应蛋白前体和脂多糖结合蛋白。途径分析表明，脓毒症会影响急性炎症反应，体液水平的调节，蛋白质活化级联，体液免疫反应和吞噬作用。蛋白质组学研究的生物学相关性的验证通过免疫测定显着增加了触珠蛋白，C反应蛋白，α1酸性糖蛋白和铜蓝蛋白的浓度。母犬中的脓毒症已显示出对包括急性期蛋白在内的炎性疾病的刺激刺激宿主防御系统蛋白的增加。意义：本研究中使用的基于标签的高分辨率定量蛋白质组学分析和生物信息学方法提供了深入的认识，与脓毒症相关的炎症复杂病理生理学揭示了具有生物标志物潜力的蛋白质。早期诊断和治疗干预可以预防与脓毒症发作的脓毒症相关的严重并发症。因此，在临床验证后可用于兽医实践以及与疾病反应途径相关的蛋白质的诊断生物标志物的鉴定是该研究的重要发现。数据可通过ProteomeXchange获得，其标识符为PXD015951。因此，在临床验证后可用于兽医实践和与疾病反应途径相关的蛋白质的诊断生物标志物的鉴定是该研究的重要发现。数据可通过ProteomeXchange获得，其标识符为PXD015951。因此，在临床验证后可用于兽医实践和与疾病反应途径相关的蛋白质的诊断生物标志物的鉴定是该研究的重要发现。数据可通过ProteomeXchange获得，其标识符为PXD015951。