Abstract A sensitive and rapid method was developed for the determination of free amino acid profiles in sweetpotatoes. The method utilized an ultra-high-performance liquid chromatography system with hydrophilic interaction liquid chromatography (HILIC) separation coupled with tandem mass-spectrometry (MS/MS) detection without the need for chemical derivatization. Separation of 36 amino acid standards was achieved on a silica HILIC column in a single MS/MS run of 19 min. This validated method was applied for the analysis of the free amino acid composition of five commercial sweetpotato cultivars (Covington, Beauregard, Hatteras, Murasaki-29 and O’ Henry) from two planting lots grown in experimental fields. Analysis of variance with Tukey’s test was used to determine the differences in individual free amino acid content among the analyzed samples, and principle component analysis (PCA) was implemented to evaluate the variation in amino acid profiles of different sweetpotato cultivars. Asparagine was the most abundant free amino acid and its content varied significantly among the cultivars (p

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亲水相互作用液相色谱-质谱法测定五种商品甘薯中的游离氨基酸

摘要 开发了一种灵敏、快速的方法来测定甘薯中的游离氨基酸谱。该方法利用具有亲水相互作用液相色谱 (HILIC) 分离和串联质谱 (MS/MS) 检测的超高效液相色谱系统，无需化学衍生。在硅胶 HILIC 色谱柱上在 19 分钟的单次 MS/MS 运行中实现了 36 种氨基酸标准的分离。这种经过验证的方法用于分析来自试验田的两个种植地的五种商业甘薯品种（Covington、Beauregard、Hatteras、Murasaki-29 和 O' Henry）的游离氨基酸组成。Tukey 检验方差分析用于确定分析样品中个体游离氨基酸含量的差异，实施主成分分析 (PCA) 以评估不同甘薯品种的氨基酸谱变化。天冬酰胺是含量最丰富的游离氨基酸，其含量在不同品种间存在显着差异（p