Abstract An endophytic fungus, Alternaria GFAV15 with antimicrobial potential was isolated from the green unripe fruit of Tinospora cordifolia, a well-known medicinal plant of semi-arid region of Rajasthan, India. The isolated endophytic fungus from green fruit has been identified on the basis of cultural and morphological characters and was characterized by internal transcribed spacer (ITS 1 and ITS 4) sequence analysis. The fungus sequence has been submitted at NCBI with accession number MK212342 with the name Alternaria GFAV15. Thin-layer chromatography (TLC) guided bioautography was performed to localize and unlock the antimicrobial bioactive compounds within the isolated fungal extract. Methyl esters (hexadecanoic acid and octadecanoic acid) were identified and recorded by GC-MS (gas chromatography-mass spectrometry) as the major compounds in active sub-fraction (RF 0.257). These compounds showed strong antibacterial activity in combination against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative).

中文翻译：

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半干旱地区Tinospora cordifolia (Willd.) Miers绿色果实内生真菌Alternaria GFAV15的分离与表征

摘要 从印度拉贾斯坦邦半干旱地区著名的药用植物心叶青霉(Tinospora cordifolia)的绿色未成熟果实中分离出一种具有抗菌潜力的内生真菌Alternaria GFAV15。从绿色水果中分离出的内生真菌已根据培养和形态特征进行鉴定，并通过内部转录间隔区（ITS 1 和 ITS 4）序列分析进行表征。该真菌序列已在 NCBI 提交，登录号为 MK212342，名称为 Alternaria GFAV15。进行薄层色谱 (TLC) 引导的生物自显影以定位和解锁分离的真菌提取物中的抗菌生物活性化合物。甲酯（十六烷酸和十八烷酸）通过 GC-MS（气相色谱-质谱）被鉴定和记录为活性亚级分 (RF 0.257) 中的主要化合物。这些化合物对金黄色葡萄球菌（革兰氏阳性）和大肠杆菌（革兰氏阴性）显示出很强的抗菌活性。