当前位置: X-MOL 学术Tissue Cell › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A compound of concentrated growth factor and periodontal ligament stem cell-derived conditioned medium.
Tissue & Cell ( IF 2.6 ) Pub Date : 2020-05-13 , DOI: 10.1016/j.tice.2020.101373
Z Aghamohamadi 1 , M Kadkhodazadeh 2 , M Torshabi 3 , F Tabatabaei 4
Affiliation  

The aim of this study was to determine the in vitro effect of a compound of concentrated growth factor (CGF) and periodontal ligament stem cell-derived conditioned medium (PDLSCs-CM) as a potential product for future applications in periodontal tissue regeneration. Isolated PDLSCs were characterized using flow cytometry and differentiation into osteoblasts and adipocytes cells. PDLSCs-CM and CGF were prepared and lyophilized. To determine the optimal concentration of the CGF-CM compound, the proliferation of PDLSCs after exposure to a wide range of different concentrations of CGF, CM, or their combination (CGF + CM) was investigated by methyl thiazol tetrazolium assay. Successful isolation of PDLSCs was confirmed by high expression of mesenchymal surface markers and differentiation into osteoblasts and adipocytes. PDLSCs showed higher proliferation in the 6.25 mg/mL concentration of CM and 94 μg/mL concentration of CGF. High concentrations of CGF and CM markedly inhibited the proliferation of PDLCs (p < 0.05). The exposure of PDLSCs to the compound of 10% CM + 90% CGF significantly increased the cell proliferation (p < 0.05). The results showed that CGF, CM, or their combination exert a proliferative effect on cells at a certain concentration. Further investigation on the synergistic effect of this compound may approve its application for periodontal regeneration.



中文翻译:

浓缩生长因子和牙周膜干细胞衍生条件培养基的化合物。

这项研究的目的是确定体外浓缩生长因子(CGF)和牙周膜干细胞条件培养液(PDLSCs-CM)的化合物作为潜在产品在牙周组织再生中的潜在作用。使用流式细胞仪表征分离的PDLSC,并分化为成骨细胞和脂肪细胞。制备PDLSCs-CM和CGF并冻干。为了确定CGF-CM化合物的最佳浓度,通过甲基噻唑四唑鎓分析法研究了暴露于各种不同浓度的CGF,CM或它们的组合(CGF + CM)后PDLSC的增殖。通过高表达间充质表面标志物并分化为成骨细胞和脂肪细胞,证实成功分离了PDLSCs。PDLSCs在6。浓度为25 mg / mL的CM和浓度为94μg/ mL的CGF。高浓度的CGF和CM显着抑制PDLC的增殖(p<0.05)。将PDLSCs暴露于10%CM + 90%CGF的化合物中可显着增加细胞增殖(p <0.05)。结果表明,CGF,CM或它们的组合在一定浓度下对细胞产生增殖作用。对该化合物的协同作用的进一步研究可能会批准其在牙周再生中的应用。

更新日期:2020-05-13
down
wechat
bug