Cryopreservation is a safe approach for the long-term preservation of plant genetic resources that complements traditional field and in vitro germplasm collections. There are many reports of cryopreservation successes in the literature; however, only a fraction of those methods has been routinely used for long-term conservation. A common problem is the difficulty of transferring technology and validating protocols from one laboratory to another. There are many critical factors involved including: the quality and type of plant materials, pretreatment and preculture conditions, cryopreservation method, cooling, storage and warming conditions, and regrowth assessment methods. In addition, adequate facilities and trained personnel are needed. Herein, we present practical aspects with regard to the challenges in implementing plant shoot tip cryopreservation technologies in cryo-banks.

低温保存是长期保存植物遗传资源的一种安全方法，可补充传统的田间和体外种质资源。文献中有许多关于冷冻保存成功的报道。但是，这些方法中只有一小部分被常规用于长期保存。一个常见的问题是难以将技术和验证协议从一个实验室转移到另一个实验室。涉及许多关键因素，包括：植物材料的质量和类型，预处理和预培养条件，冷冻保存方法，冷却，储存和加热条件以及再生评估方法。另外，需要足够的设施和训练有素的人员。在这里