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Bioethanol from hydrolyzed Spirulina ( Arthrospira platensis ) biomass using ethanologenic bacteria
Bioresources and Bioprocessing ( IF 4.6 ) Pub Date : 2020-05-12 , DOI: 10.1186/s40643-020-00315-9 Eliana B. Werlang , Jennifer Julich , Maria V. G. Muller , Fabio de Farias Neves , Estefanía Sierra-Ibarra , Alfredo Martinez , Rosana de C. de S. Schneider
Bioresources and Bioprocessing ( IF 4.6 ) Pub Date : 2020-05-12 , DOI: 10.1186/s40643-020-00315-9 Eliana B. Werlang , Jennifer Julich , Maria V. G. Muller , Fabio de Farias Neves , Estefanía Sierra-Ibarra , Alfredo Martinez , Rosana de C. de S. Schneider
Photosynthetic microorganisms are considered excellent feedstock for biofuel production in developing biomass production technologies. A study was conducted to evaluate ethanol production with the sequential enzymatic saccharification and fermentation of Arthrospira platensis (Spirulina) biomass with the metabolically engineered Escherichia coli strain MS04. A. platensis was cultivated semicontinuously in an open raceway pond, and the carbohydrate content was determined to be as high as 40%. The enzymatic saccharification was designed to release the maximum amount of glucose. After 40 h of enzymatic saccharification, 27 g L−1 of monosaccharides was obtained. These slurries were fermented with ethanologenic bacteria, achieving 12.7 g L−1 ethanol after 9 h of fermentation, which corresponds to 92% conversion yield of the glucose content in the hydrolysate, 0.13 g of ethanol per 1 g of Spirulina biomass and a volumetric productivity of 1.4 g of ethanol L−1 h−1. Therefore, we conclude that it is possible, in a short time, to obtain a high ethanol yield corresponding to 160 L per ton of dry biomass with a high productivity.
中文翻译:
利用产乙醇菌水解螺旋藻(Arthrospira platensis)生物质产生的生物乙醇。
在发展的生物质生产技术中,光合微生物被认为是生产生物燃料的极佳原料。进行了一项研究,以评估通过顺序酶促糖化和代谢工程改造的大肠杆菌MS04菌株钝顶螺旋藻(Spirulina)生物量的乙醇生产。在开阔的水道池塘中半连续种植A. platensis,确定其碳水化合物含量高达40%。设计酶促糖化以释放最大量的葡萄糖。酶促糖化40小时后,27 g L -1获得单糖。将这些浆液用产乙醇细菌发酵,发酵9小时后获得12.7 g L -1乙醇,相当于水解液中葡萄糖含量的转化率为92%,每1 g螺旋藻生物质中0.13 g乙醇和体积生产率1.4克乙醇L -1 h -1。因此,我们得出结论,有可能在短时间内以高生产率获得相当于每吨干生物质160 L的高乙醇收率。
更新日期:2020-05-12
中文翻译:
利用产乙醇菌水解螺旋藻(Arthrospira platensis)生物质产生的生物乙醇。
在发展的生物质生产技术中,光合微生物被认为是生产生物燃料的极佳原料。进行了一项研究,以评估通过顺序酶促糖化和代谢工程改造的大肠杆菌MS04菌株钝顶螺旋藻(Spirulina)生物量的乙醇生产。在开阔的水道池塘中半连续种植A. platensis,确定其碳水化合物含量高达40%。设计酶促糖化以释放最大量的葡萄糖。酶促糖化40小时后,27 g L -1获得单糖。将这些浆液用产乙醇细菌发酵,发酵9小时后获得12.7 g L -1乙醇,相当于水解液中葡萄糖含量的转化率为92%,每1 g螺旋藻生物质中0.13 g乙醇和体积生产率1.4克乙醇L -1 h -1。因此,我们得出结论,有可能在短时间内以高生产率获得相当于每吨干生物质160 L的高乙醇收率。