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Dressing up artificial viral capsids self-assembled from C-terminal-modified β-annulus peptides
Polymer Journal ( IF 2.8 ) Pub Date : 2020-05-13 , DOI: 10.1038/s41428-020-0355-4
Kazunori Matsuura

A variety of chemical approaches for the rational design of artificial proteins and peptides have been developed in recent years for the construction of self-assembled nanocapsules. It was previously found that a synthetic 24-mer β-annulus peptide, which participates in the formation of the dodecahedral internal skeleton of the tomato bushy stunt virus capsid, spontaneously self-assembled into artificial viral capsids with a size of 30–50 nm. These artificial viral capsids were established to encapsulate various guest molecules, such as anionic dyes, DNA, quantum dots, and His-tagged proteins. The artificial viral capsids could also be dressed up with gold nanoparticles, single-stranded DNA, coiled-coil spikes, and proteins by modifying with these molecules at the C-terminus of β-annulus peptides. The artificial viral capsids were notably stabilized by dressing up with human serum albumin and acquired enzymatic activity by dressing up with ribonuclease. It was found that a synthetic 24-mer β-annulus peptide, which participates in the formation of the dodecahedral internal skeleton of the tomato bushy stunt virus capsid, spontaneously self-assembled into artificial viral capsids with a size of 30–50 nm. The artificial viral capsids could be dressed up with gold nanoparticles, single-stranded DNA, coiled-coil spikes, and proteins by modifying with these molecules at the C-terminus of β-annulus peptides. The artificial viral capsids were notably stabilized by dressing up with human serum albumin and acquired enzymatic activity by dressing up with ribonuclease.

中文翻译:

装扮由 C 端修饰的 β-环肽自组装的人工病毒衣壳

近年来,人们开发了多种合理设计人工蛋白质和肽的化学方法,用于构建自组装纳米胶囊。先前发现,参与番茄丛状特技病毒衣壳十二面体内部骨架形成的合成 24-mer β-环肽自发自组装成大小为 30-50 nm 的人工病毒衣壳。建立这些人工病毒衣壳以封装各种客体分子,例如阴离子染料、DNA、量子点和带组氨酸标签的蛋白质。通过在 β-环肽的 C 端用这些分子进行修饰,人工病毒衣壳也可以用金纳米粒子、单链 DNA、卷曲螺旋尖峰和蛋白质修饰。人工病毒衣壳通过用人血清白蛋白修饰而显着稳定,并通过用核糖核酸酶修饰获得酶活性。发现参与番茄丛状特技病毒衣壳十二面体内部骨架形成的合成 24-mer β-环肽自发自组装成大小为 30-50 nm 的人工病毒衣壳。通过在 β 环肽的 C 端用这些分子进行修饰,可以用金纳米粒子、单链 DNA、卷曲螺旋尖峰和蛋白质修饰人造病毒衣壳。人工病毒衣壳通过用人血清白蛋白修饰而显着稳定,并通过用核糖核酸酶修饰获得酶活性。
更新日期:2020-05-13
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