当前位置: X-MOL 学术Int. J. Odonatol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Development and validation of microsatellite markers for an endangered dragonfly, Libellula angelina (Odonata: Libellulidae), with notes on population structures and genetic diversity
International Journal of Odonatology ( IF 0.9 ) Pub Date : 2020-01-20 , DOI: 10.1080/13887890.2019.1701573
Min Jee Kim 1 , Jong Seok Kim 1 , Sung-Soo Kim 2 , Iksoo Kim 1
Affiliation  

The Bekko Tombo, Libellula angelina Selys, 1883 (Odonata: Libellulidae), is listed as an endangered species in South Korea, and is classified as a critically endangered species by the International Union for Conservation of Nature (IUCN). An assessment of the genetic diversity and population relationships of the species by molecular markers can provide the information necessary to establish effective conservation strategies. In this study, we developed 10 microsatellite markers specific to L. angelina using the Illumina NextSeq 500 platform. Forty-three samples of L. angelina collected from three localities in South Korea were genotyped to validate these markers and to preliminarily assess the population genetic characteristics. The 10 markers revealed 4–11 alleles, 0.211–0.950 observed heterozygosity (H O), and 0.659–0.871 expected heterozygosity (H E) in the population with the largest sample size (n = 20), thereby validating the suitability of these markers for population analyses. Our preliminary assessment of the population genetic characteristics appears to indicate the following: presence of inbreeding in all populations, an isolation of the most geographically distant population (Seocheon), and a lower H O than H E. The microsatellite markers developed in this study will be useful for studying the population genetics of L. angelina collected from additional sites in South Korea and from other regions.

中文翻译:

开发和验证濒危蜻蜓 Libellula angelina(蜻蜓目:蜻蜓科)的微卫星标记,并附有关于种群结构和遗传多样性的说明

Bekko Tombo, Libellula angelina Selys, 1883 (Odonata: Libellulidae),在韩国被列为濒危物种,并被国际自然保护联盟 (IUCN) 列为极度濒危物种。通过分子标记评估物种的遗传多样性和种群关系可以提供建立有效保护策略所需的信息。在本研究中,我们使用 Illumina NextSeq 500 平台开发了 10 个特定于安吉丽娜的微卫星标记。对从韩国三个地区采集的 43 个安吉丽娜样本进行基因分型,以验证这些标记并初步评估种群遗传特征。10 个标记显示 4-11 个等位基因、0.211-0.950 观察到的杂合性 (H2O) 和 0.659-0。871 预期杂合性 (HE) 在具有最大样本量 (n = 20) 的群体中,从而验证了这些标记对群体分析的适用性。我们对种群遗传特征的初步评估似乎表明:所有种群都存在近亲繁殖,地理上最远的种群(舒川)的隔离,以及比 HE 低的 H2O。本研究中开发的微卫星标记将是有助于研究从韩国和其他地区的其他地点收集的安吉丽娜的种群遗传学。
更新日期:2020-01-20
down
wechat
bug