ABSTRACT

In spite of increase in milk production, dairy farmers suffer huge economic losses due to mastitis even in very well organized and modern farms. It has been observed that with the increase in the ability of milk production, dairy animals become more susceptible to mastitis. One of the most common causes of mastitis is infection of the mammary gland caused by bacteria like Staphylococcus aureus and Escherichia coli responsible for invoking sub-clinical and clinical mastitis, respectively. The resistivity or susceptibility of species/breeds towards mastitis depends on mode and intensity of immune response to invading pathogens. Inflammation of the mammary tissue results in the release of several cytokines, among which release of interleukin 10 (IL-10) has been identified as an important cytokine for the control of immune response. The present study was undertaken to evaluate the level of IL-10 expression when Peripheral Blood Mononuclear Cells (PBMCs) isolated from Crossbred cattle, Tharparkar cattle and Murrah buffalo were stimulated by antigens obtained from Staphylococcus aureus and Escherichia coli. The results demonstrated deviations in the level of IL-10 expression among these breeds and species in response to the stimulation by the same dose of antigens.

Abbreviation: CMT (California Mastitis Test); ELISA (Enzyme-Linked Immunosorbent Assay); LPS (Lipopolysaccharide); LTA (Lipoteichoic acid); PBMCs (Peripheral Blood Mononuclear cells); PGN (Peptidoglycan); SCC (Somatic Cell Count)

摘要

尽管牛奶产量增加，但即使在组织良好的现代化农场中，奶农仍因乳腺炎而遭受巨大的经济损失。据观察，随着产奶能力的提高，奶牛变得更容易患乳腺炎。乳腺炎最常见的原因之一是由金黄色葡萄球菌和大肠杆菌等细菌引起的乳腺感染分别负责引起亚临床和临床乳腺炎。物种/品种对乳腺炎的抵抗力或易感性取决于对入侵病原体的免疫反应模式和强度。乳腺组织的炎症导致几种细胞因子的释放，其中白细胞介素 10 (IL-10) 的释放已被确定为控制免疫反应的重要细胞因子。本研究旨在评估从杂交牛、Tharparkar 牛和 Murrah 水牛分离的外周血单个核细胞 (PBMC) 受到金黄色葡萄球菌和大肠杆菌的抗原刺激时 IL-10 的表达水平. 结果表明，这些品种和物种之间的 IL-10 表达水平在对相同剂量抗原刺激的反应中存在差异。

缩写：CMT（加利福尼亚乳腺炎试验）；ELISA（酶联免疫吸附试验）；LPS（脂多糖）；LTA（脂磷壁酸）；PBMC（外周血单核细胞）；PGN（肽聚糖）；SCC（体细胞计数）