Abstract

Traditionally, sheep are raised in Egypt for meat and wool production. Selection for higher wool quality could increase its suitability for particular processing procedures in the wool industry, which maximizes the profit of the sheep production enterprise. In this research, the effect of genetic polymorphisms of the keratin-associated protein 6-1 (KAP6-1) on wool traits was investigated in Barki sheep. Animals were genotyped by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP). Results identified a single nucleotide polymorphism (rs589531301, G > C) with three genotypes (GG, GC and CC) and the genotypic frequencies were 14.63, 43.82 and 41.55%, respectively. The KAP6-1 genotypes significantly (p > 0.05) affected greasy fleece weight (GFW; p = 0.05), prickle factor (PF; p = 0.02), staple length (SL; p = 0.038), fiber diameter (FD; p = 0.015), kemp score (KS; p = 0.048), greasy color grade (GCG; p = 0.037), luster grade (LS; p = 0.048) and the greasy structure (GS; p = 0.038). The noteworthy, animals with the CC genotype produce more wool (GFW = 1.116 kg) with longer SL (9.03 cm), finer wool (FD = 24.34 μm) and higher KS compared to other genotypes. The results of this report presented the KAP6-1 gene as a candidate gene to improve the wool production traits in the Egyptian Barki sheep and worldwide.

摘要

传统上，埃及饲养绵羊用于生产肉类和羊毛。选择更高质量的羊毛可以增加其对羊毛行业特定加工程序的适用性，从而使绵羊生产企业的利润最大化。在这项研究中，研究了角蛋白相关蛋白 6-1 (KAP6-1) 的遗传多态性对 Barki 羊羊毛性状的影响。通过聚合酶链反应 (PCR)-单链构象多态性 (SSCP) 对动物进行基因分型。结果鉴定出具有三种基因型（GG、GC 和 CC）的单核苷酸多态性（rs589531301，G > C），基因型频率分别为 14.63、43.82 和 41.55%。KAP6-1 基因型显着 ( p  > 0.05) 影响油腻羊毛重量 (GFW; p = 0.05)、刺痛系数 (PF; p  = 0.02)、短纤维长度 (SL; p  = 0.038)、纤维直径 (FD; p  = 0.015)、kemp 评分 (KS; p  = 0.048)、油脂颜色等级 (GCG; p  = 0.037)、光泽等级 (LS; p  = 0.048) 和油腻结构 (GS; p  = 0.038)。值得注意的是，与其他基因型相比，具有 CC 基因型的动物产生更多的羊毛（GFW = 1.116 kg）、更长的 SL（9.03 cm）、更细的羊毛（FD = 24.34 μm）和更高的 KS。该报告的结果将KAP6-1基因作为改善埃及 Barki 绵羊和全世界羊毛生产性状的候选基因。