Abstract

In this study, embryos of Sepiella japonica from eye primordium formation to the larval growing stage were collected and used for RNA-Seq analysis. A total of 183,542,186 clean reads were assembled de novo into 58,054 unigenes consisting of 54,118,228 bp, with the average length at 932 bp and the N50 at 1667 bp. 21,469 (36.98%) unigenes were annotated at least in one of four databases including non-redundant protein (NR), Swiss-Prot, clusters of orthologous groups of proteins (KOG) and Kyoto Encyclopedia of Genes and Genomes (KEGG). 4460 (7.68%) unigenes were annotated in all databases. Analysis of differentially expressed genes (DEGs) was carried out on embryos at Eye primordium formation stage (SJ1), organ differentiation stage (SJ2), and hatching stage (SJ3). Overall, the current study provided the de novo assembly of S. japonica transcriptome and identified the DEGs and pathways during embryonic development, which will provide a fundamental genetic resource for further functional research.

摘要

在本研究中，粳稻胚胎收集从眼原基形成到幼虫生长阶段的数据并用于 RNA-Seq 分析。总共 183,542,186 个干净读数从头组装成 58,054 个 unigenes，由 54,118,228 bp 组成，平均长度为 932 bp，N50 为 1667 bp。21,469 (36.98%) 个 unigenes 至少在四个数据库之一中被注释，包括非冗余蛋白质 (NR)、Swiss-Prot、直系同源蛋白质群 (KOG) 和京都基因和基因组百科全书 (KEGG)。在所有数据库中注释了 4460 (7.68%) 个 unigenes。在眼原基形成阶段（SJ1）、器官分化阶段（SJ2）和孵化阶段（SJ3）对胚胎进行差异表达基因（DEGs）分析。总体而言，目前的研究提供了S. japonica 的从头组装 转录组并鉴定胚胎发育过程中的DEGs和途径，这将为进一步的功能研究提供基础遗传资源。