Abstract

This study was undertaken to investigate the effect of temperature (25 °C and 30 °C) and nitrogen supplementation (with 20 g/L peptone or without) on bioethanol production from waste bread, watermelon and muskmelon feedstocks by Saccharomyces cerevisiae. Nitrogen addition resulted in higher ethanol yields at both temperatures. Fermentation at 30 °C produced a higher ethanol yield than at 25 °C. The highest ethanol yield of 0.502 gethanol /g fermentable sugar, at 98.22% of the theoretical maximum, was obtained from the fermentation of muskmelon juice at 30 °C with nitrogen supplementation. Ethanol yields at both temperatures were significantly different (p < 0.05) between the trials with nitrogen and without nitrogen supplementation for watermelon and muskmelon but not for waste bread hydrolysate. On the other hand, ethanol yields showed significant differences (p < 0.05) at 25 °C and 30 °C for all feedstocks with nitrogen and without supplementation.

摘要

本研究旨在调查温度（25°C 和 30°C）和氮补充（添加或不添加蛋白胨）对酿酒酵母从废弃面包、西瓜和甜瓜原料中生产生物乙醇的影响。在两个温度下，氮的添加导致更高的乙醇产率。在 30°C 下发酵产生的乙醇产量高于 25°C。甜瓜汁在 30 °C 下加氮发酵获得的最高乙醇产量为 0.502 g乙醇/g 可发酵糖，为理论最大值的 98.22%。两种温度下的乙醇产量显着不同（p < 0.05) 对西瓜和甜瓜进行加氮和不加氮试验之间，但对于废面包水解物则没有。另一方面， 在 25 °C 和 30 °C 下，所有含氮和未添加的原料的乙醇产量均显示出显着差异 ( p < 0.05)。