Abstract

The depletion of non-renewable fossil fuels and a surge in their prices have led researchers to seek alternative, renewable energy sources; among them, biodiesel is a good option, especially in the ever-growing global transport sector. Mustard oil is a potential feedstock for biodiesel production, and because it is non-edible it avoids the food versus fuel feud. Biocatalytic transesterification of mustard oil for biodiesel production makes this process cost efficient, environmentally friendly and sustainable. In the current study, Pseudomanas aeruginosa Q8 KX12304-mediated transesterification of mustard oil was carried out. The parameters for the transesterification reaction were statistically optimized using Plackett–Burman and central composite design. The highest biodiesel volumetric yield obtained was 100% at optimized conditions. The quality of biodiesel was confirmed using gas chromatography–mass spectrometry (GCMS) and the produced biodiesel was found to meet the quality standards specified by American Society for Testing and Materials (ASTM) and EU-14103. The fatty acid methyl ester contents of the biodiesel produced were erucic acid methyl esters (48.2%), palmitic acid methyl esters (11.8%), oleic acid methyl esters (10.6%), linolenic acid methyl esters (10.3%), linoleic acid methyl esters (9.1%) and stearic acid methyl esters (8%). To the best of our knowledge, this is the first study to report transesterification of mustard oil via biocatalysis.

摘要

不可再生化石燃料的枯竭及其价格的飙升促使研究人员寻求替代的可再生能源；其中，生物柴油是一个不错的选择，尤其是在不断增长的全球运输部门。芥子油是生物柴油生产的潜在原料，因为它不可食用，所以避免了食物与燃料之间的争执。芥子油用于生物柴油生产的生物催化酯交换使该过程具有成本效益、环境友好和可持续性。在目前的研究中，铜绿假单胞菌进行了芥子油的 Q8 KX12304 介导的酯交换。使用 Plackett-Burman 和中心复合设计对酯交换反应的参数进行统计优化。在优化条件下获得的最高生物柴油体积产量为 100%。生物柴油的质量通过气相色谱-质谱联用 (GCMS) 确认，生产的生物柴油符合美国材料试验协会 (ASTM) 和 EU-14103 规定的质量标准。生产的生物柴油的脂肪酸甲酯含量为芥酸甲酯（48.2%）、棕榈酸甲酯（11.8%）、油酸甲酯（10.6%）、亚麻酸甲酯（10.3%）、亚油酸甲酯酯 (9.1%) 和硬脂酸甲酯 (8%)。据我们所知，